APH1A in Human Brain. APH1A was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human APH1A Monoclonal Antibody (Catalog # MAB6908) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Anterior pharynx-defective 1 (APH1A) is a widely expressed 36 kDa 7-transmembrane protein found in the endoplasmic reticulum and Golgi. APH1A is a component of the gamma secretase proteolytic complex which cleaves multiple substrates within their transmembrane domains. This activity liberates the intracellular domains of ErbB4, Neuregulin-1, and Notch receptors in addition to the A-beta peptide from the amyloid precursor protein (APP). Alternate splicing generates a short isoform of human APH1A that lacks 18 amino acids at the C-terminus. Within aa 58-69, human APH1A shares 100% aa sequence identity with mouse and rat APH1A.
gamma-Secretase Subunit Anterior Pharynx Defective 1 Homolog A
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