Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 665404
View all APH1A Primary Antibodies »

Product Specifications

Immunogen

Human APH1A synthetic peptide
SSFERFEIFPKHVTDRSDARLQY
Accession # Q96BI3

Specificity

Detects human APH1A in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human APH1A Antibody

APH1A antibody in Human Brain by Immunohistochemistry (IHC-P).

APH1A in Human Brain.

APH1A was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human APH1A Monoclonal Antibody (Catalog # MAB6908) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human APH1A Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APH1A

Anterior pharynx-defective 1 (APH1A) is a widely expressed 36 kDa 7-transmembrane protein found in the endoplasmic reticulum and Golgi. APH1A is a component of the gamma secretase proteolytic complex which cleaves multiple substrates within their transmembrane domains. This activity liberates the intracellular domains of ErbB4, Neuregulin-1, and Notch receptors in addition to the A-beta peptide from the amyloid precursor protein (APP). Alternate splicing generates a short isoform of human APH1A that lacks 18 amino acids at the C-terminus. Within aa 58-69, human APH1A shares 100% aa sequence identity with mouse and rat APH1A.

Long Name

gamma-Secretase Subunit Anterior Pharynx Defective 1 Homolog A

Alternate Names

6530402N02Rik, anterior pharynx defective 1 homolog A (C. elegans), APH-1, APH-1A, Aph-1alpha, CGI-78, gamma-secretase subunit APH-1A, Presenilin-stabilization factor, PSF

Entrez Gene IDs

51107 (Human); 100039623 (Mouse)

Gene Symbol

APH1A

UniProt

Q96BI3

Additional APH1A Products

Product Documents for Human APH1A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human APH1A Antibody

For research use only

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Protocols

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