Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all APLP-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human APLP-1
Gln34-Glu580
Accession # P51693

Specificity

Detects human APLP‑1 in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse APLP-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human APLP‑1 Antibody

APLP‑1 in Human Brain.

APLP‑1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human APLP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3129) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using out protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human and Mouse APLP‑1 by Simple WesternTM.

Simple Western lane view shows lysates of human brain (hippocampus) and mouse brain (hippocampus), loaded at 0.2 mg/mL. A specific band was detected for APLP‑1 at approximately 119 kDa (as indicated) using 25 µg/mL of Goat Anti-Human APLP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3129). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human APLP‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

0.3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human APLP‑1, see our available Western blot detection antibodies

Simple Western

25 µg/mL
Sample: Human brain (hippocampus) and mouse brain (hippocampus)

Western Blot

0.1 µg/mL
Sample: Recombinant Human APLP‑1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APLP-1

APLP-1 is a transmembrane metalloprotein that is expressed in central neurons. Similar to APP and APLP-2, APLP-1 is susceptible to cleavage by various secretases, generating multiple fragments from the extracellular and intracellular domains. These include peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that associates with Fe65 family proteins and functions as a transcriptional activator. The extracellular domain contains heparin and collagen binding regions and is 89% identical between human and mouse.

Long Name

Amyloid-like Protein 1

Alternate Names

APLP1

Entrez Gene IDs

333 (Human); 11803 (Mouse); 502317 (Rat)

Gene Symbol

APLP1

UniProt

P51693

Additional APLP-1 Products

Product Documents for Human APLP‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human APLP‑1 Antibody

For research use only

Citations for Human APLP‑1 Antibody

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Protocols

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