Human APLP-1 Antibody

Catalog # Availability Size / Price Qty
APLP‑1 in Human Brain.
2 Images
Product Details
Citations (1)
Supplemental Products

Human APLP-1 Antibody Summary

Species Reactivity
Detects human APLP‑1 in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse APLP-1 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human APLP-1
Accession # P51693
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human APLP‑1
Simple Western
25 µg/mL
Human brain (hippocampus) and mouse brain (hippocampus)
0.3-15 µg/mL
Immersion fixed paraffin-embedded sections of human brain (cortex)
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human APLP‑1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

APLP‑1 in Human Brain. APLP‑1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human APLP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3129) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using out protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western View Larger

Detection of Human and Mouse APLP‑1 by Simple WesternTM. Simple Western lane view shows lysates of human brain (hippocampus) and mouse brain (hippocampus), loaded at 0.2 mg/mL. A specific band was detected for APLP‑1 at approximately 119 kDa (as indicated) using 25 µg/mL of Goat Anti-Human APLP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3129). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APLP-1

APLP-1 is a transmembrane metalloprotein that is expressed in central neurons. Similar to APP and APLP-2, APLP-1 is susceptible to cleavage by various secretases, generating multiple fragments from the extracellular and intracellular domains. These include peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that associates with Fe65 family proteins and functions as a transcriptional activator. The extracellular domain contains heparin and collagen binding regions and is 89% identical between human and mouse.

Long Name
Amyloid-like Protein 1
Entrez Gene IDs
333 (Human); 11803 (Mouse); 502317 (Rat)
Alternate Names
amyloid beta (A4) precursor-like protein 1; amyloid precursor-like protein 1; amyloid-like protein 1; APLP1; APLP-1; APLPAPLP-1

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Citation for Human APLP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. APLP1 is endoproteolytically cleaved by ?-secretase without previous ectodomain shedding
    Authors: L Schauenbur, F Liebsch, M Eravci, MC Mayer, C Weise, G Multhaup
    Sci Rep, 2018;8(1):1916.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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