Human Apolipoprotein E/ApoE Antibody Summary
Accession # P02649
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Apolipoprotein E/ApoE by Western Blot. Western blot shows lysates of human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Apolipoprotein E/ApoE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4144) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Apolipoprotein E/ApoE at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Apolipoprotein E/ ApoE by Western Blot. Western blot shows conditioned media from SK-Mel-28 human malignant melanoma cell line, HepG2 human hepatocellular carcinoma cell line, and human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Apolipoprotein E/ApoE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4144) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Apolipoprotein E/ApoE at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Apolipoprotein E/ ApoE by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 1:10 dilution. A specific band was detected for Apolipoprotein E/ApoE at approximately 39 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Apolipoprotein E/ApoE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4144) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein E/ApoE
ApoE is a major protein component of serum LDL, VLDL, HDL, and chylomicrons. It is produced predominantly by hepatocytes, macrophages, and non-neuronal cells in the CNS. ApoE-containing particles transport triglycerides and cholesterol to peripheral tissues for cellular uptake and catabolism (1‑4). Mature human ApoE is a 37 kDa glycoprotein that consists of an N-terminal domain composed of four bundled alpha -helices, plus a hinge region and an extended alpha -helical C-terminal domain (2, 5). Its amphipathic nature and flexible structure enables it to adopt dramatically different conformations upon lipid association (2). ApoE is monomeric in lipid particles, although it forms oligomers when lipid-free (6). ApoE3 is the most abundant of the three common alleles in human; ApoE2 and ApoE4 differ by single aa substitutions (1). Mature human ApoE shares 71% aa sequence identity with mouse and rat ApoE. LDL receptor family proteins preferentially bind and internalize the lipid-bound form of ApoE with the exception of VLDLR which also efficiently internalizes lipid-free ApoE (7, 8). Lipoprotein uptake is facilitated by the initial binding of ApoE to cell surface heparan sulfate proteoglycans (HSPG) (9). Receptor/HSPG binding and lipid interactions primarily involve the N- and C-terminal regions of ApoE, respectively (2). Recycled lipid-free ApoE is formed into HDL particles through interactions with the lipid transporter ABCA1 (10). High cellular sterol content activates the nuclear hormone receptor LXR which promotes increased ApoE synthesis and increased sterol efflux, while low sterol content induces LDL R expression with increased sterol uptake and decreased ApoE production (11). ApoE3 dampens the TNF-alpha induced inflammatory response in vascular endothelial cells (12). In the CNS, ApoE blocks production of the amyloid A beta peptide by inhibiting the gamma -secretase cleavage of APP (13). It also complexes with A beta and promotes A beta internalization via LRP2 (14, 15).
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- Chroni, A. et al. (2005) Biochemistry 44:13132.
- Futamura, M. et al. (2005) J. Biol. Chem. 280:5414.
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- Lucic, D. et al. (2007) J. Lipid Res. 48:366.
- Mullick, A.E. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:339.
- Irizarry, M.C. et al. (2004) J. Neurochem. 90:1132.
- Naslund, J. et al. (1995) Neuron 15:219.
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Citations for Human Apolipoprotein E/ApoE Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Serum Apolipoprotein E and Other Inflammatory Markers Can Identify Non-Responding Patients to a Dendritic Cell Vaccine
Authors: H Leeman, E Kaminska, D Green, M Bodman-Smi, A Gravett, K Bodman-Smi, J Copier, G Coulton, A Fusi, AG Dalgleish
Transl Oncol, 2019;12(3):397-403.
Sample Types: Serum
Applications: ELISA Development (Detection)
MicroRNA-27a regulates lipid metabolism and inhibits hepatitis C virus replication in human hepatoma cells.
Authors: Shirasaki T, Honda M, Shimakami T, Horii R, Yamashita T, Sakai Y, Sakai A, Okada H, Watanabe R, Murakami S, Yi M, Lemon S, Kaneko S
J Virol, 2013;87(9):5270-86.
Sample Types: Cell Lysates
Applications: Western Blot
Can Human Apolipoprotein E/ApoE Antibody, Catalog # AF4144, detect all forms, ApoE2, ApoE3 and ApoE4?
Yes. This antibody can detect ApoE2, ApoE3 and ApoE4. Although we have not specifically tested each of the three separately, given the high homology between the three forms, we would expect this polyclonal antibody to detect multiple epitopes on each of the three forms.
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