Human Arylsulfatase A/ARSA Antibody

• Species Reactivity
  Human
• Specificity
  Detects human ARSA in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse ARSA is observed.
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  Mouse myeloma cell line NS0-derived recombinant human ARSA
  Arg19-Ala507
  Accession # AAH14210
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.1 µg/mL
  Recombinant Human Arylsulfatase A/ARSA (Catalog # 2485-SU)
• Immunohistochemistry
  5-15 µg/mL
  See below
• Immunoprecipitation
  25 µg/mL
  Conditioned cell culture medium spiked with Recombinant Human Arylsulfatase A/ARSA (Catalog # 2485-SU), see our available Western blot detection antibodies

Immunohistochemistry click image to view larger

Arylsulfatase A/ARSA in Human Liver. Arylsulfatase A/ARSA was detected in immersion fixed paraffin-embedded sections of human liver using 1.7 µg/mL Human Arylsulfatase A/ARSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2485) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Lukatela, G. et al. (1998) Biochemistry 37:3654.
  2. Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
  3. Stein, C. et al. (1989) J. Biol. Chem. 264:1252.

• Entrez Gene IDs:
  410 (Human); 11883 (Mouse)

• Alternate Names:
  ARSA; Arylsulfatase A; ASA; Cerebroside 3-sulfatase; Cerebroside-sulfatase; EC 3.1.6; EC 3.1.6.8; MLD

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