Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Arylsulfatase A/ARSA Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ARSA
Arg19-Ala507
Accession # AAH14210

Specificity

Detects human ARSA in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant mouse ARSA is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Arylsulfatase A/ARSA Antibody

Arylsulfatase A/ARSA antibody in Human Liver by Immunohistochemistry (IHC-P).

Arylsulfatase A/ARSA in Human Liver.

Arylsulfatase A/ARSA was detected in immersion fixed paraffin-embedded sections of human liver using 1.7 µg/mL Human Arylsulfatase A/ARSA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2485) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Arylsulfatase A/ARSA Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Arylsulfatase A/ARSA (Catalog # 2485-SU), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Arylsulfatase A/ARSA (Catalog # 2485-SU)

Reviewed Applications

Read 1 review rated 4 using AF2485 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Arylsulfatase A/ARSA

As a member of the sulfatase family, ARSA is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet (1). The ARSA deficiency results in metachromatic leukodystrophy (MLD), a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms (2). The deduced amino acid sequence of human ARSA consists of a signal peptide (residues 1-18) and a mature chain (residues 19-507) (3). Recombinant human ARSA corresponds to the mature chain and has sulfatase activity described above.

References

  1. Lukatela, G. et al. (1998) Biochemistry 37:3654.
  2. Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
  3. Stein, C. et al. (1989) J. Biol. Chem. 264:1252.

Alternate Names

ARSA, Cerebroside 3-sulfatase

Entrez Gene IDs

410 (Human); 11883 (Mouse)

Gene Symbol

ARSA

UniProt

AAH14210

Additional Arylsulfatase A/ARSA Products

Product Documents for Human Arylsulfatase A/ARSA Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Arylsulfatase A/ARSA Antibody

For research use only

Citations for Human Arylsulfatase A/ARSA Antibody

Customer Reviews for Human Arylsulfatase A/ARSA Antibody (1)

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  • Human Arylsulfatase A/ARSA Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPS2 human induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 04/30/2019

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Protocols

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