Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2403C
View all BAFFR/TNFRSF13C Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line, NS0-derived human BAFF R/TNFRSF13C
Ser7-Ala71
Accession # Q96RJ3

Specificity

Detects human BAFF R/TNFRSF13C in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human BAFFR/TNFRSF13C MAb (Clone 2403C)

Detection of BAFF R/TNFRSF13C antibody in PBMCs antibody by Flow Cytometry.

Detection of BAFF R/TNFRSF13C in PBMCs by Flow Cytometry.

Peripheral blood mononuclear cells (PBMCs) were stained with either (A) Rabbit Anti-Human BAFF R/TNFRSF13C Monoclonal Antibody (Catalog # MAB1162) or (B) Rabbit IgG Control Antibody (Catalog # MAB1050) followed by APC-conjugated anti-rabbit IgG secondary antibody (Catalog # F0111) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). View our protocol for Staining Membrane-associated Proteins.
BAFF R/TNFRSF13C antibody in Human Spleen by Immunohistochemistry (IHC-P).

BAFF R/TNFRSF13C in Human Spleen.

BAFF R/TNFRSF13C was detected in immersion fixed paraffin-embedded sections of human spleen using Rabbit Anti-Human BAFF R/TNFRSF13C Monoclonal Antibody (Catalog # MAB1162) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human BAFFR/TNFRSF13C MAb (Clone 2403C)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human PBMC

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Reviewed Applications

Read 3 reviews rated 4.3 using MAB1162 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BAFFR/TNFRSF13C

B-cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation (1-4). BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells. Similarly, A/WySnJ mice that are defective in BAFF-R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B-cell development. While the function of BCMA is not defined, TACI has been shown to control B-cell homeostasis and T-cell-independent immune responses.

References

  1. Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
  2. Mackay F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
  3. Laabi, Y. et al. (2001) Current Biol. 11:R1013.
  4. Thompson, J.S. et al. (2001) Science 14:2108.

Long Name

B cell Activating Factor Receptor

Alternate Names

BAFF R, BR3, CD268, TNFRSF13C

Entrez Gene IDs

115650 (Human); 72049 (Mouse)

Gene Symbol

TNFRSF13C

UniProt

Q96RJ3

Additional BAFFR/TNFRSF13C Products

Product Documents for Human BAFFR/TNFRSF13C MAb (Clone 2403C)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BAFFR/TNFRSF13C MAb (Clone 2403C)

For research use only

Customer Reviews for Human BAFFR/TNFRSF13C MAb (Clone 2403C) (3)

4.3 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human BAFFR/TNFRSF13C Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Pancreatic Ductal Adenocarcinoma
    Species: Human
    Verified Customer | Posted 11/11/2021
    Human BAFFR/TNFRSF13C MAb (Clone 2403C) MAB1162
  • Human BAFFR/TNFRSF13C Antibody
    Name: Lydia Taverne
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human spleen, FFPE
    Species: Human
    Verified Customer | Posted 12/16/2019
    Spleen tissue stained with 1:50 MAB1162 shows strong membranous B-cell positivity (red fluorescence) in white pulp, but no positive staining in red pulp.
    Citrate buffer (pH 6) antigen unmasking. Antibody was used at a 1:50 concentration and detected with anti-rabbit AF546 (red fluor) secondary Ab.
    Human BAFFR/TNFRSF13C MAb (Clone 2403C) MAB1162
  • Human BAFFR/TNFRSF13C Antibody
    Name: L Taverne
    Application: Immunohistochemistry
    Sample Tested: Spleen tissue
    Species: Human
    Verified Customer | Posted 11/04/2019
    Fluorescent immunohistochemistry with red-labelled secondary antibody on human spleen tissue, at 1:50 dilution. Next time would use higher dilution.
    Human BAFFR/TNFRSF13C MAb (Clone 2403C) MAB1162

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Protocols

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