Human beta -NGF Antibody Summary
Accession # CAA36832
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Human beta -NGF Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human beta ‑NGF ELISA Standard Curve. Recombinant Human beta ‑NGF protein (256-GF) was serially diluted 2-fold and captured by Mouse Anti-Human beta ‑NGF Monoclonal Antibody (Catalog # MAB2561) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human beta ‑NGF Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF256) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody. Recombinant Human beta -NGF (Catalog # 256-GF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta -NGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human beta -NGF Monoclonal Antibody (Catalog # MAB2561). The ND50is typically 0.4-2.4 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.
NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.
Citations for Human beta -NGF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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The role of nerve growth factor in hyperosmolar stress induced apoptosis.
Authors: Chang EJ, Im YS, Kay EP
J. Cell. Physiol., 2008;216(1):69-77.
Sample Types: Whole Tissue
Nerve growth factor beta/pro-nerve growth factor and their receptors in normal human oral mucosa.
Authors: Hayashi K, Storesund T, Schreurs O
Eur. J. Oral Sci., 2007;115(5):344-54.
Sample Types: Whole Cells
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