Human BRCA2 Antibody
Human BRCA2 Antibody Summary
Met1-Thr200
Accession # P51587
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human BRCA2 by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human BRCA2 Monoclonal Antibody (Catalog # MAB2476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BRCA2 at approximately 380 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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BRCA2 in Human Breast Cancer Tissue. BRCA2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human BRCA2 Monoclonal Antibody (Catalog # MAB2476) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in epithelial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BRCA2
BRCA2 is a nuclear protein that acts as a tumor suppressor. Germline mutation of BRCA2 accounts for many cases of familial breast and ovarian cancer. At the cellular level, BRCA2 is involved in homologous recombination (HR), a form of double-strand break repair. BRCA2 interacts with the Rad51 recombinase, another protein critical for HR, to preserve chromosomal integrity.
Product Datasheets
Citations for Human BRCA2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Pharmacological ascorbate induces 'BRCAness' and enhances the effects of Poly(ADP-Ribose) polymerase inhibitors against BRCA1/2 wild-type ovarian cancer
Authors: Y Ma, P Chen, JA Drisko, D Khabele, AK Godwin, Q Chen
Oncol Lett, 2020;19(4):2629-2638.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
FoxM1-dependent RAD51 and BRCA2 signaling protects idiopathic pulmonary fibrosis fibroblasts from radiation-induced cell death
Authors: J Im, J Lawrence, D Seelig, RS Nho
Cell Death Dis, 2018;9(6):584.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
IGH/MYC Translocation Associates with BRCA2 Deficiency and Synthetic Lethality to PARP1 Inhibitors
Authors: S Maifrede, K Martin, P Podszywalo, K Sullivan, SK Langer, R Nejadi, Y Dasgupta, M Hulse, D Gritsyuk, M Nieborowsk, LN Lupey-Gree, H Zhao, K Piwocka, MA Wasik, I Tempera, T Skorski
Mol. Cancer Res., 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cataloging antineoplastic agents according to their effectiveness against platinum-resistant and platinum-sensitive ovarian carcinoma cell lines
Authors: K Ishiguro, YL Zhu, ZP Lin, PG Penketh, K Shyam, R Zhu, RP Baumann, AC Sartorelli, TJ Rutherford, ES Ratner
J Transl Sci, 2016;2(2):117-124.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
RAS promotes tumorigenesis through genomic instability induced by imbalanced expression of Aurora-A and BRCA2 in midbody during cytokinesis.
Authors: Yang G, Mercado-Uribe I, Multani A, Sen S, Shih I, Wong K, Gershenson D, Liu J
Int J Cancer, 2013;133(2):275-85.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Aurora kinase A promotes ovarian tumorigenesis through dysregulation of the cell cycle and suppression of BRCA2.
Authors: Yang G, Chang B, Yang F
Clin. Cancer Res., 2010;16(12):3171-81.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
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Where do you observe staining when using Human BRCA2 antibody, Catalog # MAB2476, in IHC?
Human BRCA2 staining may be observed in both the nucleus and cytoplasm.
Isotype Controls
Reconstitution Buffers
Secondary Antibodies
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