Detection of Human BRCA2 by Western Blot.
Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human BRCA2 Monoclonal Antibody (Catalog # MAB2476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BRCA2 at approximately 380 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BRCA2 is a nuclear protein that acts as a tumor suppressor. Germline mutation of BRCA2 accounts for many cases of familial breast and ovarian cancer. At the cellular level, BRCA2 is involved in homologous recombination (HR), a form of double-strand break repair. BRCA2 interacts with the Rad51 recombinase, another protein critical for HR, to preserve chromosomal integrity.
Breast Cancer 2
Entrez Gene IDs:
BRCA1/BRCA2-containing complex, subunit 2; BRCA2; BRCC2; breast and ovarian cancer susceptibility gene, early onset; breast cancer 2 tumor suppressor; breast cancer 2, early onset; breast cancer susceptibility protein BRCA2; breast cancer type 2 susceptibility protein; BROVCA2; FACD; FAD; FAD1; FANCB; FANCD; FANCD1Fanconi anemia, complementation group D1; Fanconi anemia group D1 protein; GLM3; PNCA2
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Gene expression and mutation-guided synthetic lethality eradicates proliferating and quiescent leukemia cells
Authors: M Nieborowsk, K Sullivan, Y Dasgupta, P Podszywalo, G Hoser, S Maifrede, E Martinez, D Di Marcant, E Bolton-Gil, K Cramer-Mor, J Lee, M Li, A Slupianek, D Gritsyuk, S Cerny-Reit, I Seferynska, T Stoklosa, L Bullinger, H Zhao, V Gorbunova, K Piwocka, P Valent, CI Civin, M Muschen, JE Dick, JC Wang, S Bhatia, R Bhatia, K Eppert, MD Minden, SM Sykes, T Skorski
J. Clin. Invest., 2017;127(6):2392-2406.
Sample Type: Cell Lysates
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