Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1062028
View all BTNL8 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide Fc-cleaved human BTNL8 

Specificity

Detects human BTNL8 in direct ELISA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human BTNL8 Antibody

Detection of Human BTNL8 by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidney cells transfected with human BTNL8. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB113641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for BTNL8 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of BTNL8 in Human Colon.

BTNL8 was detected in immersion fixed paraffin-embedded sections of Human Colon using Mouse Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB113641) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in lymphocytes in lamina propria. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human BTNL8 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Colon

Western Blot

2 µg/mL
Sample: HEK293T human embryonic kidney cells transfected with human BTNL8.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BTNL8

Butyrophilin-like 8 (BTNL8) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL8 is a 500 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a short cytoplasmic domain (2). The ECD of human BTNL8 shares 88% sequence identity with the ECD of mouse BTNL8. BTNL8 has two alternatively spliced forms: B7-like and BTN-like. Both isoforms of BTNL8 are expressed in a range of human tissues (3). The complete immunological function of BTNL molecules is only beginning to emerge. BTNL8 has been shown to be important in initiation of primary immune responses, suggesting a role in priming of naïve T lymphocytes (3). Down-regulation of BNTL8 mRNA levels has been associated with ulcerative colitis and colon cancer (4). BTNL8 are expressed in colon, lung, testis and neutrophils, and its expression is significantly decreased in ulcerative colitis, colonic tumors as compared to unaffected tissue (4). Soluble BTNL8-Fc fusion protein binds to resting, but not activated T cells. In vitro, BTNL8 co-stimulates T cell proliferation and cytokine production. In vivo injections of BTNL8-Fc significantly increases production of Ag‑specific IgG during the primary but not the secondary immune response (3).

References

  1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
  2. Arnett, H.A. et al. (2009) Cytokine 46:370.
  3. Chapoval, A.I. et al. (2013) Mol Immunol. 56:819.
  4. Lebrero-Fernández C. et al. (2016) Immun Inflamm Dis. 4:191.

Long Name

Butyrophilin-like 8

Alternate Names

BTN9.2

Entrez Gene IDs

79908 (Human); 406160 (Rat)

Gene Symbol

BTNL8

Additional BTNL8 Products

Product Documents for Human BTNL8 Antibody

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Product Specific Notices for Human BTNL8 Antibody

For research use only

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Protocols

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