Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 836701
View all Calponin 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Calponin 1
Ser2-Ala297
Accession # P51911

Specificity

Detects human Calponin 1 in ELISAs. Detects human, mouse and rat Calponin 1 in Western Blots. In direct ELISAs, no cross-reactivity with recombinant human Calponin 3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human Calponin 1 Antibody

Detection of Human, Mouse, and Rat Calponin 1 antibody by Western Blot.

Detection of Human, Mouse, and Rat Calponin 1 by Western Blot.

Western blot shows lysates of MDA-MB-231 human breast cancer cell line, MCF-7 human breast cancer cell line, NMuMG mouse mammary gland epithelial cell line, and A7r5 rat thoracic aortic smooth muscle cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Calponin 1 Monoclonal Antibody (Catalog # MAB7900) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Calponin 1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Calponin 1 antibody in MDA-MD-231 Human Cell Line by Immunocytochemistry (ICC).

Calponin 1 in MDA‑MD‑231 Human Cell Line.

Calponin 1 was detected in immersion fixed MDA-MD-231 human breast cancer cell line using Mouse Anti-Human Calponin 1 Monoclonal Antibody (Catalog # MAB7900) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Calponin 1 antibody in Human Small Intestine by Immunohistochemistry (IHC-P).

Calponin 1 in Human Small Intestine.

Calponin 1 was detected in immersion fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human Calponin 1 Monoclonal Antibody (Catalog # MAB7900) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Calponin 1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MDA‑MD‑231 human breast cancer cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human small intestine

Western Blot

1 µg/mL
Sample: MDA‑MB‑231 human breast cancer cell line, MCF‑7 human breast cancer cell line, NMuMG mouse mammary gland epithelial cell line, and A7r5 rat thoracic aortic smooth muscle cell line

Reviewed Applications

Read 1 review rated 5 using MAB7900 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Calponin 1

CNN-1 (Calponin 1 [calcium and calmodulin-binding troponin T-like protein]; also Calponin basic, CaP and Calponin H1) is a 32-36 kDa cytoplasmic member of the calponin family of proteins. Although reportedly expressed in fibroblasts and endothelial cells, it actually appears to be restricted to smooth muscle and smooth muscle-like cells such as myoepithelium and myofibroblasts in the adult. CNN-1 interacts with F-actin in a phosphorylation-dependent manner. When nonphosphorylated, CNN-1 blocks actomyosin ATPase activity, contributing to the stabilization of actin stress fiber bundles. Thus, CNN-1 expression inhibits cell motility and the formation of podosomes. Human CNN-1 is 297 amino acids (aa) in length. It contains one CH/calponin homology domain (aa 30-127), and three consecutive calponin-like repeats (aa 164-268). The repeats are suggested to mediate actin binding. There are five potential Ser/Thr phosphorylation sites. Full-length human CNN-1 shares 97% aa sequence identity with mouse CNN-1.

Alternate Names

Calponin H1, CNN1, Sm-Calp, SMCC

Entrez Gene IDs

1264 (Human); 12797 (Mouse); 65204 (Rat)

Gene Symbol

CNN1

UniProt

P51911 (Human)

Additional Calponin 1 Products

Product Documents for Human Calponin 1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Calponin 1 Antibody

For research use only

Customer Reviews for Human Calponin 1 Antibody (1)

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  • Human Calponin 1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Myofibroblasts
    Species: Human
    Verified Customer | Posted 12/06/2021
    Human Calponin 1 Antibody MAB7900

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Protocols

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