Detection of Human Carbonic Anhydrase X/CA10 by Western Blot. Western blot shows lysates of human brain (hippocampus) tissue. PVDF membrane was probed with 1.5 µg/mL of Mouse Anti-Human Carbonic Anhydrase X/CA10 Monoclonal Antibody (Catalog # MAB2189) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Carbonic Anhydrase X/CA10 at approximately 38‑42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase X/CA10
CA10 (Carbonic Anhydrase-related X), also called CARP X and Cerebral protein 15, is a cytosolic 35 kDa (predicted) member of the a-carbonic anhydrase family of molecules. Although it does not possess CA enzymatic activity due to the lack of Zn and His in its (potential) catalytic site, the recombinant protein used as an immunogen does show esterase activity. CA10 is found principally in CNS neurons and respiratory epithelium. Human CA10 is 328 amino acids (aa) in length. It possesses one carbonic anhydrase domain that contains a utilized phosphorylation site at Thr299. One potential isoform is reported that shows a 22 aa substitution for aa 277-328. Human and mouse CA10 are identical in amino acid sequence.
Entrez Gene IDs:
CA10; Carbonic Anhydrase X; carbonic anhydrase-related protein 10; Carbonic anhydrase-related protein X; CARP X; CARPX; CA-RPX; CARPXCA-RP X; Cerebral protein 15; cerebral protein-15; HUCEP-15
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