Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 862319
View all Carbonic Anhydrase X/CA10 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase X/CA10
Met1-Asn300
Accession # Q9NS85

Specificity

Detects human Carbonic Anhydrase X/CA10 in ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Carbonic Anhydrase X/CA10 Antibody

Detection of Human Carbonic Anhydrase X/CA10 antibody by Western Blot.

Detection of Human Carbonic Anhydrase X/CA10 by Western Blot.

Western blot shows lysates of human brain (hippocampus) tissue. PVDF membrane was probed with 1.5 µg/mL of Mouse Anti-Human Carbonic Anhydrase X/CA10 Monoclonal Antibody (Catalog # MAB2189) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Carbonic Anhydrase X/CA10 at approximately 38-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Carbonic Anhydrase X/CA10 Antibody

Application
Recommended Usage

Western Blot

1.5 µg/mL
Sample: Human brain (hippocampus) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbonic Anhydrase X/CA10

CA10 (Carbonic Anhydrase-related X), also called CARP X and Cerebral protein 15, is a cytosolic 35 kDa (predicted) member of the a-carbonic anhydrase family of molecules. Although it does not possess CA enzymatic activity due to the lack of Zn and His in its (potential) catalytic site, the recombinant protein used as an immunogen does show esterase activity. CA10 is found principally in CNS neurons and respiratory epithelium. Human CA10 is 328 amino acids (aa) in length. It possesses one carbonic anhydrase domain that contains a utilized phosphorylation site at Thr299. One potential isoform is reported that shows a 22 aa substitution for aa 277-328. Human and mouse CA10 are identical in amino acid sequence.

Alternate Names

CA10, CARPX, HUCEP-15

Entrez Gene IDs

56934 (Human)

Gene Symbol

CA10

UniProt

Q9NS85

Additional Carbonic Anhydrase X/CA10 Products

Product Documents for Human Carbonic Anhydrase X/CA10 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Carbonic Anhydrase X/CA10 Antibody

For research use only

Citations for Human Carbonic Anhydrase X/CA10 Antibody

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