Human Carboxylesterase 2/CES2 Antibody

Catalog # Availability Size / Price Qty
AF5657
AF5657-SP
Detection of Human Carboxylesterase 2/CES2 by Western Blot.
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Human Carboxylesterase 2/CES2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carboxylesterase 2/CES2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human CES1, recombinant mouse (rm) CES2, rmCES3, and rmCES5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carboxylesterase 2/CES2
Gln27-Leu559
Accession # O00748
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human liver

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Carboxylesterase 2/CES2 by Western Blot. View Larger

Detection of Human Carboxylesterase 2/CES2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NCI‑N87 human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carboxylesterase 2/CES2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5657) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carboxylesterase 2/CES2 at approximately 60‑65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxylesterase 2/CES2

CES2 (Carboxylesterase 2; also CE-2) is a glycosylated, monomeric member of the type-B carboxyesterease/lipase family of enzymes. Although it runs at 59‑62 kDa in SDS-PAGE, Gel filtration assigns an 80 kDa MW to it. CES2 is an ER luminal enzyme that catalyzes the conversion of carboxlic ester to alcohol. It is found in small intestine and liver, and likely participates in the detoxification of drugs. Mature human CES2 is 533 amino acids (aa) in length (aa 27-559). It contains a catalytic site composed of Ser228Glu345His457, and an ER-retention motif over aa 556 - 559. Individually or in combination, splice variants may show an alternate start site 64 aa upstream of the standard site, a Val substitution for aa 458-474, or a nine aa insertion after Met1. Over the length of the precursor (aa 1-559) human CES2 shares 72% aa identity with mouse CES2.

Entrez Gene IDs
8824 (Human); 234671 (Mouse); 171118 (Rat)
Alternate Names
carboxylesterase 2 (intestine, liver); Carboxylesterase 2; carboxylesterase 2EC 3.1.1.1; CE-2; CE-2PCE-2; CES2; CES2A1; EC 3.1.1; EC 3.1.1.84; hCE-2; iCE; intestinal carboxylesterase; liver carboxylesterase-2; PCE-2

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Cell and Tissue Staining Kits

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Secondary Antibodies

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