Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 89228
View all Cardiotrophin-1/CT-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cardiotrophin‑1/CT‑1
Ser2-Ala201
Accession # Q16619

Specificity

Detects human Cardiotrophin‑1/CT‑1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Cardiotrophin-1/CT-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Cardiotrophin‑1/CT‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Cardiotrophin‑1/CT‑1 (Catalog # 612-CD)

Reviewed Applications

Read 1 review rated 5 using MAB260 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cardiotrophin-1/CT-1

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third  alpha receptor subunit has been implicated in the receptor complex.

References

  1. Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81.
  2. Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.

Alternate Names

Cardiotrophin1, CTF1

Entrez Gene IDs

1489 (Human); 13019 (Mouse)

Gene Symbol

CTF1

UniProt

Q16619

Additional Cardiotrophin-1/CT-1 Products

Product Documents for Human Cardiotrophin‑1/CT‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Cardiotrophin‑1/CT‑1 Antibody

For research use only

Related Research Areas

IL-6 Family
Obesity

Citations for Human Cardiotrophin‑1/CT‑1 Antibody

Customer Reviews for Human Cardiotrophin‑1/CT‑1 Antibody (1)

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  • Human Cardiotrophin-1/CT-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Whole Cells
    Species: Human
    Verified Customer | Posted 11/18/2021
    Human Cardiotrophin‑1/CT‑1 Antibody MAB260

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