Human Casein Kinase 2 alpha Antibody

• Species Reactivity
  Human
• Specificity
  Detects human Casein Kinase 2 alpha in ELISAs and Western blots. Detects mouse and rat Casein Kinase 2 alpha in Western blots
• Source
  Monoclonal Mouse IgG₁ Clone # 844720
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  E. coli-derived recombinant human Casein Kinase 2 alpha
  Asp253-Gln391
  Accession # P68400
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  1 µg/mL
  See below
• Simple Western
  10 µg/mL
  See below
• Immunohistochemistry
  5-25 µg/mL
  See below
• Immunocytochemistry
  8-25 µg/mL
  See below

Western Blot click image to view larger	Detection of Human, Mouse, and Rat Casein Kinase 2 alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, C6 rat glioma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Casein Kinase 2 alpha at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry click image to view larger	Casein Kinase 2 alpha in HEK293 Human Cell Line. Casein Kinase 2 alpha was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western click image to view larger	Detection of Human and Mouse Casein Kinase 2 alpha by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.5 mg/mL. A specific band was detected for Casein Kinase 2 alpha at approximately 50-52 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Immunohistochemistry click image to view larger	Casein Kinase 2 alpha in Human Brain. Casein Kinase 2 alpha was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reconstitution
  Sterile PBS to a final concentration of 0.5 mg/mL.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Entrez Gene IDs:
  1457 (Human); 12995 (Mouse); 116549 (Rat)

• Alternate Names:
  Casein Kinase 2 alpha; casein kinase 2, alpha 1 polypeptide; casein kinase II alpha 1 subunit; CK II alpha; CK2 catalytic subunit alpha; CK2A1; CK2A1casein kinase II subunit alpha; CKII; CSNK2A1; EC 2.7.11; EC 2.7.11.1; protein kinase CK2

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