Detection of Human, Mouse, and Rat Casein Kinase 2 alpha by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, C6 rat glioma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Casein Kinase 2 alpha at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Casein Kinase 2 alpha in HEK293 Human Cell Line.
Casein Kinase 2 alpha was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human and Mouse Casein Kinase 2 alpha by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.5 mg/mL. A specific band was detected for Casein Kinase 2 alpha at approximately 50-52 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Casein Kinase 2 alpha in Human Brain.
Casein Kinase 2 alpha was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human Casein Kinase 2 alpha Monoclonal Antibody (Catalog # MAB7957) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Casein Kinase 2 alpha
Casein kinase 2 (CK2) is a ubiquitous and constitutively active tetrameric serine/threonine kinase that is comprised of two catalytic subunits (CK2 alpha and/or CK2 alpha ‘) and two identical regulatory subunits (CK2 beta ). CK2 has been implicated in numerous cellular processes, including signal transduction, transcription, translation, replication, and metabolic pathways. CK2 is known to phosphorylate more than 300 different substrates. Phosphorylation of cell-cycle proteins such as p53, p34cdc2, p27KIP1, and MDM-2 account for the ability of CK2 to induce proliferation, while the phosphorylation of HS1, Bid, and Max account for its antiapoptotic role. The human CK2 alpha and CK2 alpha ‘ subunits are the products of two different genes. They have highly conserved catyalytic domains but divergent C-terminal regions. Within aa 253-391 (including the region of divergence between CK2 alpha and CK2 alpha ’), the 35-45 kDa human CK2 alpha shares 96% aa sequence identity with mouse and rat CK2 alpha. An alternatively spliced isoform of human CK2 alpha lacks the N-terminal 136 amino acids including a portion of the kinase domain. CK2 beta plays dual roles in the regulation of CK2 acivity. Its C-terminal domain is responsible for stable interactions with the catalytic subunit and increased catalytic activity following tetramer formation, while the N-terminal domain exerts negative regulation on the catalytic activity of CK2.
Entrez Gene IDs:
1457 (Human); 12995 (Mouse); 116549 (Rat)
Casein Kinase 2 alpha; casein kinase 2, alpha 1 polypeptide; casein kinase II alpha 1 subunit; CK II alpha; CK2 catalytic subunit alpha; CK2A1; CK2A1casein kinase II subunit alpha; CKII; CSNK2A1; EC 2.7.11; EC 18.104.22.168; protein kinase CK2
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