Human Caspase-1 Antibody

Catalog # Availability Size / Price Qty
MAB62152-100
MAB62152-SP
Caspase‑1 in THP‑1 Human Cell Line.
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Product Details
Citations (1)
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Human Caspase-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Caspase-1 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1021323
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Caspase-1 peptide
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of human pancreas
Immunocytochemistry
8-25 µg/mL
Immersion fixed THP‑1 human acute monocytic leukemia cell line treated with PMA, LPS, and Nigericin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Caspase‑1 in THP‑1 Human Cell Line. Caspase‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line treated with PMA, LPS, and Nigericin (positive staining) and MCF‑7 human breast cancer cell line (negative control) using Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62152) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry View Larger

Caspase‑1 in Human Pancreas. Caspase‑1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Caspase‑1 Monoclonal Antibody (Catalog # MAB62152) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-1

Caspase-1, also known as IL-1 beta -converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits. Within the large subunit, human Caspase 1 shares 61% aa sequence identity with mouse and rat Caspase-1.

Entrez Gene IDs
834 (Human)
Alternate Names
CASP1 nirs variant 1; CASP1; CASP-1; caspase 1, apoptosis-related cysteine peptidase; Caspase-1 subunit p10; Caspase-1 subunit p20; Caspase1; Caspase-1; EC 3.4.22.36; EC:3.4.22.36; ICE; IL-1 beta-converting enzyme; IL1BC; IL-1BC; IL1BCE; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45

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Citation for Human Caspase-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CARD-only proteins regulate in�vivo inflammasome responses and ameliorate gout
    Authors: S Devi, M Indramohan, E Jäger, J Carriere, LH Chu, L de Almeida, DR Greaves, C Stehlik, A Dorfleutne
    Cell Reports, 2023-03-16;42(3):112265.
    Species: Human
    Sample Types: Whole Cells
    Applications: Proximity Ligation Assay

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