Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody

R&D Systems | Catalog # AF1049

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cathepsin A/Lysosomal Carboxypeptidase A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin A/Lysosomal Carboxypeptidase A
Ala29-Tyr480
Accession # P10619

Specificity

Detects both pro and active forms of human and mouse Cathepsin A/Lysosomal Carboxypeptidase A in direct ELISAs and Western blots. In Western blots, it recognizes all forms of recombinant human Cathepsin A: single chain (55 kDa), heavy chain (32 kDa), and light chain (20 kDa). Also in Western blots, less than 1% cross-reactivity with recombinant human (rh) Cathepsin B, rhCathepsin C, rhCathepsin D, rhCathepsin L, rhCathepsin L2/V, rhCathepsin O, rhCathepsin S and rhCathepsin X/Z/P is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody

Cathepsin A/Lysosomal Carboxypeptidase A antibody in Human Prostate by Immunohistochemistry (IHC-P).

Cathepsin A/Lysosomal Carboxypeptidase A in Human Prostate.

Cathepsin A/Lysosomal Carboxypeptidase A was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Cathepsin A/Lysosomal Carboxypeptidase A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1049) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Cathepsin A/Lysosomal Carboxypeptidase A (Catalog # 1049-SE)

Reviewed Applications

Read 1 review rated 4 using AF1049 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cathepsin A/Lysosomal Carboxypeptidase A

Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1). Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH. Also known as protective protein, its association with beta -galactosidase ( beta -gal) and neuraminidase is essential for beta -gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of Cathepsin A causes the lysosomal storage disorder galactosialidosis, characterized by a combined secondary deficiency of beta -gal and neuraminidase. Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including tachykinins, indicating that extralysosomal Cathepsin A plays a role in regulation of functions of these molecules (2). Cathepsin A is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds.

References

  1. Pshezhetsky, A.V. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 1923, Academic Press, San Diego.
  2. Hiraiwa, M. (1999) Cell. Mol. Life. Sci. 56:894.

Alternate Names

CTSA, Lysosomal Carboxypeptidase A

Entrez Gene IDs

5476 (Human); 19025 (Mouse)

Gene Symbol

CTSA

UniProt

P10619

Additional Cathepsin A/Lysosomal Carboxypeptidase A Products

Product Documents for Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody

Certificate of Analysis

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Product Specific Notices for Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody

For research use only

Citations for Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody

Customer Reviews for Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody (1)

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  • Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: B103 lysate and SVG-A lysate
    Species: Human and Rat
    Verified Customer | Posted 08/30/2018
    CHAP lysates from the indicated cell lines, loaded at the indicated concentrations. Antibody was tested at 1:25 dil.
    Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody AF1049

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