Human Cathepsin C/DPPI Antibody

Catalog # Availability Size / Price Qty
AF1071
AF1071-SP
Detection of Human Cathepsin C/DPPI by Western Blot.
3 Images
Product Details
Citations (6)
FAQs
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Human Cathepsin C/DPPI Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cathepsin C/DPPI in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human pro Cathepsin C/DPPI
Asp25-Leu463
Accession # P53634
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Cathepsin C/DPPI antibody by Western Blot. View Larger

Detection of Human Cathepsin C/DPPI by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1071) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cathepsin C/DPPI at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Cathepsin C/DPPI antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Cathepsin C/DPPI in Human Lung Cancer Tissue. Cathepsin C/DPPI was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1071) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin C/DPPI

Cathepsin C, also known as dipeptidyl-peptidase I (DPPI), is a cysteine protease of the papain family (1). Cathepsin C sequentially removes dipeptides from the free N-termini of proteins and peptides. It has broad specificity except that it does not cleave a basic amino acid (Arg or Lys) in the N-terminal position or Pro on either side of the scissle bond. It requires halide ions for activity. The pro form contains a pro peptide and a catalytic region, which can be further processed into heavy/ alpha and light/ beta chains that are linked by a disulfide bond. It is broadly distributed. Cathepsin C plays a role in the lysosomal degradation. It also functions as a key enzyme in the activation of granule serine proteases in cytotoxic T lymphocytes and natural killer cells (granzymes A and B), mast cells (tryptase and chymase), and neutrophils (Cathepsin G and elastase) by removing their N-terminal activation dipeptides (2). Loss of function mutations in the Cathepsin C gene result in periodontal disease and palmoplantar keratosis (3).

References
  1. Turk, B. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 1192, Academic Press, San Diego.
  2. Dahl, S.W. et al. (2001) Biochemistry 40:1671.
  3. Toomes, A.J. et al. (1999) Nat. Genet. 23:421.
Entrez Gene IDs
1075 (Human); 13032 (Mouse); 25423 (Rat)
Alternate Names
Cathepsin C; cathepsin CEC 3.4.14.1; Cathepsin J; CPPIHMS; CTSC; dipeptidyl peptidase 1; Dipeptidyl peptidase I; Dipeptidyl transferase; dipeptidyl-peptidase I; DPP1; DPPI; DPP-I; JP; JPD; PALS; PLS

Product Datasheets

Citations for Human Cathepsin C/DPPI Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Mutations in MAGT1 lead to a glycosylation disorder with a variable phenotype
    Authors: E Blommaert, R Péanne, NA Cherepanov, D Rymen, F Staels, J Jaeken, V Race, L Keldermans, E Souche, A Corveleyn, R Sparkes, K Bhattachar, C Devalck, R Schrijvers, F Foulquier, R Gilmore, G Matthijs
    Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases
    Authors: Y Hamon, M Legowska, V Hervé, S Dallet-Cho, S Marchand-A, L Vanderlynd, M Demonte, R Williams, CJ Scott, M Si-Tahar, N Heuzé-Vour, G Lalmanach, DE Jenne, A Lesner, F Gauthier, B Korkmaz
    J. Biol. Chem., 2016;291(16):8486-99.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Promiscuous processing of human alphabeta-protryptases by cathepsins L, B, and C.
    Authors: Le QT, Min HK, Xia HZ, Fukuoka Y, Katunuma N, Schwartz LB
    J. Immunol., 2011;186(12):7136-43.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  4. Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
    Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
    Blood, 2007;111(4):2339-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Distinct roles for cysteine cathepsin genes in multistage tumorigenesis.
    Authors: Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA
    Genes Dev., 2006;20(5):543-56.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.
    Authors: Cherepanova N, Shrimal S, Gilmore R
    J Cell Biol, 0;206(4):525-39.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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