Human Cathepsin C/DPPI Antibody
Human Cathepsin C/DPPI Antibody Summary
Asp25-Leu463
Accession # P53634
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Detection of Human Cathepsin C/DPPI by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1071) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cathepsin C/DPPI at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Cathepsin C/DPPI in Human Lung Cancer Tissue. Cathepsin C/DPPI was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human Cathepsin C/DPPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1071) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin C/DPPI
Cathepsin C, also known as dipeptidyl-peptidase I (DPPI), is a cysteine protease of the papain family (1). Cathepsin C sequentially removes dipeptides from the free N-termini of proteins and peptides. It has broad specificity except that it does not cleave a basic amino acid (Arg or Lys) in the N-terminal position or Pro on either side of the scissle bond. It requires halide ions for activity. The pro form contains a pro peptide and a catalytic region, which can be further processed into heavy/ alpha and light/ beta chains that are linked by a disulfide bond. It is broadly distributed. Cathepsin C plays a role in the lysosomal degradation. It also functions as a key enzyme in the activation of granule serine proteases in cytotoxic T lymphocytes and natural killer cells (granzymes A and B), mast cells (tryptase and chymase), and neutrophils (Cathepsin G and elastase) by removing their N-terminal activation dipeptides (2). Loss of function mutations in the Cathepsin C gene result in periodontal disease and palmoplantar keratosis (3).
- Turk, B. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 1192, Academic Press, San Diego.
- Dahl, S.W. et al. (2001) Biochemistry 40:1671.
- Toomes, A.J. et al. (1999) Nat. Genet. 23:421.
Product Datasheets
Citations for Human Cathepsin C/DPPI Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Mutations in MAGT1 lead to a glycosylation disorder with a variable phenotype
Authors: E Blommaert, R Péanne, NA Cherepanov, D Rymen, F Staels, J Jaeken, V Race, L Keldermans, E Souche, A Corveleyn, R Sparkes, K Bhattachar, C Devalck, R Schrijvers, F Foulquier, R Gilmore, G Matthijs
Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Neutrophilic Cathepsin C Is Maturated by a Multistep Proteolytic Process and Secreted by Activated Cells during Inflammatory Lung Diseases
Authors: Y Hamon, M Legowska, V Hervé, S Dallet-Cho, S Marchand-A, L Vanderlynd, M Demonte, R Williams, CJ Scott, M Si-Tahar, N Heuzé-Vour, G Lalmanach, DE Jenne, A Lesner, F Gauthier, B Korkmaz
J. Biol. Chem., 2016;291(16):8486-99.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Promiscuous processing of human alphabeta-protryptases by cathepsins L, B, and C.
Authors: Le QT, Min HK, Xia HZ, Fukuoka Y, Katunuma N, Schwartz LB
J. Immunol., 2011;186(12):7136-43.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
Blood, 2007;111(4):2339-46.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Distinct roles for cysteine cathepsin genes in multistage tumorigenesis.
Authors: Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA
Genes Dev., 2006;20(5):543-56.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.
Authors: Cherepanova N, Shrimal S, Gilmore R
J Cell Biol, 0;206(4):525-39.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
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