Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Primate

Cited:

Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Transgenic Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry, Neutralization, Immunocytochemistry, Bioassay, ELISA Capture, ELISA Development, ELISA Microarray Development, In vivo assay, Functional Assay, Luminex Development, Mass Spectrometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 21418
View all CCL5/RANTES Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL5/RANTES

Specificity

Detects human and primate CCL5/RANTES in direct ELISAs and Western blots. When used in combination with the biotinylated anti-human/primate CCL5/RANTES detection antibody (Catalog # BAF278) in sandwich ELISAs, no significant cross-reactivity or interference is observed with recombinant human (rh) ANG, rhCNTF, rh beta -ECGF, rhEGF, mouse (m) EGF, rhEpo, bovine (b) FGF acidic, rhFGF acidic, bFGF basic, rhFGF basic, rhFGF-4, rhG-CSF, rhGM-CSF, recombinant mouse (rm) GM-CSF, rhGRO alpha, rhIFN-gamma, rhIGF-I, rhIGF-II, rhIL-1 alpha, rmIL-1 alpha, rhIL-1 beta, rmIL‑1 beta, rhIL-1ra, recombinant rat (rr) IL-1ra, rhIL-2, rhIL-3, rmIL-3, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL‑10, rhIL-11, rhLIF, rhMCP‑1, rhM-CSF, rhMIP-1 alpha, rmMIP-1 alpha, rhMIP-1 beta, rmMIP-1 beta, rhOSM, human (h) PDGF, pPDGF, rhPDGF-AA, rhPDGF‑AB, rhPDGF-BB, rhSCF, rmSCF, rhSLPI, rhTGF-alpha, pTGF-beta 1, hTGF-beta 1, rhTGF-beta 1, pTGF-beta 2, rhTGF-beta 2, recombinant canine TGF-beta 3, rhTGF-beta 3, recombinant amphibian TGF-beta 5, rhTNF-alpha, rmTNF-alpha, or rmTNF-beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human/Primate CCL5/RANTES Antibody

Detection of Human CCL5/RANTES by Functional

Detection of Human CCL5/RANTES by Functional

The expression of chemokine CCL5 is up-regulated in co-cultures of BMMSCs cells and OTSCC.Co-culture of OTSCC cells with BMMSCs results in the high expression of chemokine CCL5 in HSC-3 and SAS cells, but not in dysplastic DOK cells (A). Function-blocking monoclonal antibody against CCL5 (Mab CCL5) slightly inhibits BMMSC enhanced invasion area, but has no effect on the invasion depth, whereas the antibody against CXCL1 does not increase the inhibitory effect in OTSCC invasion (B, C). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24204919), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CCL5/RANTES by Immunocytochemistry/Immunofluorescence

Detection of Human CCL5/RANTES by Immunocytochemistry/Immunofluorescence

RANTES filaments are present after exposure to shear stress.(A) HUVECs were electroporated with DNA encoding RANTES, cultivated on coverslips before stimulation with TNF alpha + IFN gamma for 30 h. RANTESwas labeled with an anti-RANTES antibody (clone 21418), and the cover slipswere mounted in a laminar flow chamber. The flow rate was adjusted to mimicvessel wall shear stress of 1 dyne/cm2. The imageswere acquired by confocal microscopy before (left image, 0 min) andafter 4 min with exposure to flow forces (right image, 4 min).(B) HUVECs were treated as described in (A) except that they were notlabeled with anti-RANTES antibody before exposure to flow. Human peripheralblood mononuclear cells were labeled with an anti-CD45 antibody, resuspendedin medium and applied by a pump to the flow chamber(1 dyne/cm2). After 10 min, the cells werelabeled with a rabbit anti-RANTES antibody. Images were acquired bysequential scanning confocal microscopy. Arrows and arrow heads indicateRANTES filaments and RANTES positive platelets, respectively. Originalmagnification in all panels, × 100. Scale bars, 10 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25791723), licensed under a CC-BY license. Not internally tested by R&D Systems.

Neutralization by Human/Primate CCL5/RANTES Antibody.

Human/Primate CCL5/RANTES Antibody (Catalog # MAB678) neutralizes Recombinant Human CCL5/RANTES (278-RN) induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) for this effect is typically 80.0-800 ng/mL in the presence of 10 ng/mL Recombinant Human CCL5/RANTES.

Applications for Human/Primate CCL5/RANTES Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CCL5/RANTES (Catalog # 278-RN)

Neutralization

Human/Primate CCL5/RANTES Antibody (Catalog # MAB678) neutralizes Recombinant Human CCL5/RANTES (Catalog # 278-RN) induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) for this effect is typically 80.0-800 ng/mL in the presence of 10 ng/mL Recombinant Human CCL5/RANTES.

Human/Primate CCL5/RANTES Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human/Primate CCL5/RANTES Biotinylated Antibody (Catalog # BAF278)
  • Standard: Recombinant Human CCL5/RANTES Protein (Catalog # 278-RN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.5 using MAB678 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL5/RANTES

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily. It binds and activates the chemokine receptors CCR1, 3 and 5.

Alternate Names

RANTES, SISd

Entrez Gene IDs

6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)

Gene Symbol

CCL5

Additional CCL5/RANTES Products

Product Documents for Human/Primate CCL5/RANTES Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Primate CCL5/RANTES Antibody

For research use only

Citations for Human/Primate CCL5/RANTES Antibody

Customer Reviews for Human/Primate CCL5/RANTES Antibody (4)

4.5 out of 5
4 Customer Ratings
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human/Primate CCL5/RANTES Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Primate
    Verified Customer | Posted 11/28/2022
    Used as a capture in our NHP ELISA
    Human/Primate CCL5/RANTES Antibody MAB678
  • Human/Primate CCL5/RANTES Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 07/24/2018
    Human/Primate CCL5/RANTES Antibody MAB678
  • Human/Primate CCL5/RANTES Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2017
  • Human/Primate CCL5/RANTES Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/22/2017
    The RANTES ELISA we made used MAG678 as the capture and BAF278 as the detection. Dilutional linearity of samples wasn't great but otherwise the assay worked very well.
    Human/Primate CCL5/RANTES Antibody MAB678

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