Human CCL5/RANTES Antibody

Catalog #: AF-278-NA
22 Citations Datasheet / COA / SDS

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AF-278-NA
AF-278-SP

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Detection of Recombinant Human and Mouse CCL5/RANTES by Western Blot.

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Citations (22)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL5/RANTES Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL5/RANTES in direct ELISAs. Detects human CCL5/RANTES and mouse CCL5/RANTES Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant canine CCL5/RANTES is observed and approximately 20% cross-reactivity with recombinant cotton rat CCL5/RANTES is observed. In Western blots, no cross-reactivity with recombinant human (rh) CCL3 and rhCCL4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CCL5/RANTES
Ser24-Ser91
Accession # P13501

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 0.1-0.4 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL5/RANTES.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Recombinant Human and Mouse CCL5/RANTES by Western Blot. Western blot shows 25 ng of Recombinant Human CCL5/RANTES (Catalog # 278-RN), Recombinant Mouse CCL5/RANTES (Catalog # 478-MR), Recombinant Human CCL3/MIP-1a Isoform LD78a (Catalog # 270-LD), and Recombinant Human CCL4/MIP-1 beta (Catalog # 271-BME). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL5/RANTES at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry

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CCL5/RANTES in Human PBMCs. CCL5/RANTES was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (orange; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

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CCL5/RANTES in Human Tonsil. CCL5/RANTES was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human CCL5/RANTES by Simple Western^TM. Simple Western lane view shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200nM PMA for 24 hrs and 10ug/mL LPS for 3 hrs, loaded at 0.2 mg/mL. A specific band was detected for CCL5/RANTES at approximately 7 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Neutralization

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Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody. Recombinant Human CCL5/RANTES (Catalog # 278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA). The ND₅₀ is typically 0.1-0.4 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL5/RANTES

RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily and is now designated CCL5. It binds and activates the chemokine receptors CCR1, 3 and 5.

Entrez Gene IDs

6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)

Alternate Names

CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CCL5/RANTES Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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The Protein Kinase Receptor Modulates the Innate Immune Response against Tacaribe Virus
Authors: H Moreno, S Kunz
Viruses, 2021;13(7):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial Cells
Authors: MC Mladinich, JN Conde, WR Schutt, SY Sohn, ER Mackow
MBio, 2021;12(4):e0196221.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma
Authors: YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang
Nature Communications, 2021;12(1):4091.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
CCR5/CCL5 axis interaction promotes migratory and invasiveness of pancreatic cancer cells
Authors: SK Singh, MK Mishra, IA Eltoum, S Bae, JW Lillard, R Singh
Sci Rep, 2018;8(1):1323.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy.
Authors: Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann I, Levine B, June C, Zhang L, Wang E, Marincola F, Bedognetti D, Powell D, Tanyi J, Feldman M, Kandalaft L, Coukos G
Clin Cancer Res, 2015;21(12):2840-50.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques.
Authors: Shang L, Smith A, Duan L, Perkey K, Qu L, Wietgrefe S, Zupancic M, Southern P, Masek-Hammerman K, Reeves R, Johnson R, Haase A
J Immunol, 2014;193(1):277-84.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHC
Lenalidomide inhibits lymphangiogenesis in preclinical models of mantle cell lymphoma.
Authors: Song K, Herzog B, Sheng M, Fu J, McDaniel J, Chen H, Ruan J, Xia L
Cancer Res, 2013;73(24):7254-64.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Expression and regulation of chemokines in murine and human type 1 diabetes.
Authors: Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
Diabetes, 2012;61(2):436-46.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype.
Authors: Ouwehand K, Oosterhoff D, Breetveld M
J. Invest. Dermatol., 2011;131(2):418-25.
Species: Human
Sample Types: Whole Tissue
Applications: Neutralization
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
J. Clin. Invest., 2010;120(3):907-23.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Disrupting functional interactions between platelet chemokines inhibits atherosclerosis in hyperlipidemic mice.
Authors: Koenen RR, von Hundelshausen P, Nesmelova IV, Zernecke A, Liehn EA, Sarabi A, Kramp BK, Piccinini AM, Paludan SR, Kowalska MA, Kungl AJ, Hackeng TM, Mayo KH, Weber C
Nat. Med., 2009;15(1):97-103.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
Authors: Renaud SJ, Sullivan R, Graham CH
Placenta, 2009;30(4):313-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
J. Immunol., 2009;182(6):3919-27.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
Authors: Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA
Am. J. Transplant., 2008;8(7):1512-22.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
J. Neurosci., 2008;28(13):3277-90.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Mesenchymal stem cells within tumour stroma promote breast cancer metastasis.
Authors: Karnoub AE, Dash AB, Vo AP, Sullivan A, Brooks MW, Bell GW, Richardson AL, Polyak K, Tubo R, Weinberg RA
Nature, 2007;449(7162):557-63.
Species: Human
Sample Types: In Vivo
Applications: Neutralization

Renal transplantation: examination of the regulation of chemokine binding during acute rejection.
Authors: Ali S, Malik G, Burns A, Robertson H, Kirby JA
Transplantation, 2005;79(6):672-9.
Species: Human
Sample Types: Whole Cells
Applications: ICC

The beta chemokines CCL4 and CCL5 enhance adhesion of specific CD4+ T cell subsets to human brain endothelial cells.
Authors: Quandt J, Dorovini-Zis K
J. Neuropathol. Exp. Neurol., 2004;63(4):350-62.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
J. Leukoc. Biol., 2003;73(5):584-90.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
Authors: Binninger-Schinzel D, 2019, Norley S, Adler HS, Oberg HH, Kurth R
, 2002;294(1):209-21.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
Authors: Sato E, Koyama S
Eur. Respir. J., 2000;16(5):951-8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
Authors: Rump L, Mattey D, Kehoe O, Middleton J
Cytokine, 0;97(0):133-140.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr