Human CCL5/RANTES Antibody

(17 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCL5/RANTES in direct ELISAs. Detects human CCL5/RANTES and mouse CCL5/RANTES Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant canine CCL5/RANTES is observed and approximately 20% cross-reactivity with recombinant cotton rat CCL5/RANTES is observed. In Western blots, no cross-reactivity with recombinant human (rh) CCL3 and rhCCL4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CCL5/RANTES
    Ser24-Ser91
    Accession # P13501
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL5/RANTES.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human and Mouse CCL5/RANTES by Western Blot. Western blot shows 25 ng of Recombinant Human CCL5/RANTES (Catalog # 278-RN), Recombinant Mouse CCL5/RANTES (Catalog # 478-MR), Recombinant Human CCL3/MIP‑1 alpha Isoform LD78a (Catalog # 270-LD), and Recombinant Human CCL4/MIP‑1 beta (Catalog # 271-BME). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-278-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL5/RANTES at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunocytochemistry
CCL5/RANTES in Human PBMCs.

CCL5/RANTES was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑278‑NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (orange; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry
CCL5/RANTES in Human Tonsil.

CCL5/RANTES was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑278‑NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody.

Recombinant Human CCL5/RANTES (Catalog # 278‑RN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL5/RANTES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑278‑NA). The ND50 is typically 0.1‑0.4 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL5/RANTES

RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily and is now designated CCL5. It binds and activates the chemokine receptors CCR1, 3 and 5.

  • Entrez Gene IDs:
    6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
  • Alternate Names:
    CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy.
    Authors: Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann I, Levine B, June C, Zhang L, Wang E, Marincola F, Bedognetti D, Powell D, Tanyi J, Feldman M, Kandalaft L, Coukos G
    Clin Cancer Res, 2015;21(12):2840-50.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  2. NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaques.
    Authors: Shang L, Smith A, Duan L, Perkey K, Qu L, Wietgrefe S, Zupancic M, Southern P, Masek-Hammerman K, Reeves R, Johnson R, Haase A
    J Immunol, 2014;193(1):277-84.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Tissue
    Application: IHC
  3. Lenalidomide inhibits lymphangiogenesis in preclinical models of mantle cell lymphoma.
    Authors: Song K, Herzog B, Sheng M, Fu J, McDaniel J, Chen H, Ruan J, Xia L
    Cancer Res, 2013;73(24):7254-64.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. Expression and regulation of chemokines in murine and human type 1 diabetes.
    Authors: Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
    Diabetes, 2012;61(2):436-46.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype.
    Authors: Ouwehand K, Oosterhoff D, Breetveld M
    J. Invest. Dermatol., 2011;131(2):418-25.
    Species: Human
    Sample Type: Whole Tissue
    Application: Neut
  6. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
    Authors: Renaud SJ, Sullivan R, Graham CH
    Placenta, 2009;30(4):313-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Disrupting functional interactions between platelet chemokines inhibits atherosclerosis in hyperlipidemic mice.
    Authors: Koenen RR, von Hundelshausen P, Nesmelova IV, Zernecke A, Liehn EA, Sarabi A, Kramp BK, Piccinini AM, Paludan SR, Kowalska MA, Kungl AJ, Hackeng TM, Mayo KH, Weber C
    Nat. Med., 2009;15(1):97-103.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  9. Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
    Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
    J. Immunol., 2009;182(6):3919-27.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  10. Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
    Authors: Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA
    Am. J. Transplant., 2008;8(7):1512-22.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
    Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
    J. Neurosci., 2008;28(13):3277-90.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  12. Mesenchymal stem cells within tumour stroma promote breast cancer metastasis.
    Authors: Karnoub AE, Dash AB, Vo AP, Sullivan A, Brooks MW, Bell GW, Richardson AL, Polyak K, Tubo R, Weinberg RA
    Nature, 2007;449(7162):557-63.
    Species: Human
    Sample Type: In Vivo
    Application: In vivo
  13. Renal transplantation: examination of the regulation of chemokine binding during acute rejection.
    Authors: Ali S, Malik G, Burns A, Robertson H, Kirby JA
    Transplantation, 2005;79(6):672-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. The beta chemokines CCL4 and CCL5 enhance adhesion of specific CD4+ T cell subsets to human brain endothelial cells.
    Authors: Quandt J, Dorovini-Zis K
    J. Neuropathol. Exp. Neurol., 2004;63(4):350-62.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  15. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003;73(5):584-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  16. Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
    Authors: Binninger-Schinzel D, Norley S, Adler HS, Oberg HH, Kurth R
    Virology, 2002;294(1):209-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  17. Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
    Authors: Sato E, Koyama S
    Eur. Respir. J., 2000;16(5):951-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Expand to show all 17 Citations
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