Detection of CCR9 in Human MOLT-4 cells by Flow Cytometry.
MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791, filled histogram) or Mouse IgG2A Flow Cytometry Isotype Control(Catalog # MAB003) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0102B).View our protocol for Staining Membrane-associated Proteins.
Detection of CCR9 in HEK293 Human Cell Line Transfected with Human CCR9 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human CCR9 and eGFP was stained with (A) Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791) or (B) Mouse IgG2A isotype control antibody (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
CCR9 in MOLT‑4 Human Cell Line.
CCR9 was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.
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