Human CCR9 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB1791R-100
MAB1791R-SP
Detection of CCR9 in Human MOLT-4 cells by Flow Cytometry.
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Human CCR9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR9 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG2A Clone # 248621R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human CCR9 transfectants
Met1-Leu369
Accession # P51686
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CCR9 antibody in Human MOLT-4 cells antibody by Flow Cytometry. View Larger

Detection of CCR9 in Human MOLT-4 cells by Flow Cytometry. MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791R, filled histogram) or Mouse IgG2AFlow Cytometry Isotype Control (Catalog # MAB003) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR9 antibody in HEK293 Human Cell Line Transfected with Human CCR9 and eGFP antibody by Flow Cytometry. View Larger

Detection of CCR9 in HEK293 Human Cell Line Transfected with Human CCR9 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human CCR9 and eGFP was stained with (A) Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791R) or (B) Mouse IgG2Aisotype control antibody (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry CCR9 antibody in MOLT-4 Human Cell Line by Immunocytochemistry (ICC). View Larger

CCR9 in MOLT‑4 Human Cell Line. CCR9 was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791R) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.

Entrez Gene IDs
10803 (Human); 12769 (Mouse); 282832 (Rat)
Alternate Names
C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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