Human CD160 Antibody

Catalog # Availability Size / Price Qty
MAB6700
MAB6700-SP

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Detection of CD160 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (1)
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Reviews (2)

Human CD160 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD160 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD160 is observed.
Source
Monoclonal Mouse IgG2B Clone # 688327
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD160
Ile27-Ser159
Accession # O95971
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD160 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD160 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD160 Monoclonal Antibody (Catalog # MAB6700) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD8a PE-conjugated Monoclonal Antibody (Catalog # FAB1509P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD160

CD160 (also BY55) is a 27 kDa member of the immunoglobulin superfamily of molecules.  It is expressed on select hematopoietic cell types, including CD56dim CD16+ cytotoxic NK cells, CD8+ CD28- effector T cells, δ/ gamma T cells, and restricted CD4+ T cells.  It is a receptor for HLA-C molecules, and its engagement induces CD160+ NK cells to both secrete IFN-gamma plus TNF-alpha and initiate a cytotoxic program.  Human CD160 was originally identified as a 155 amino acid (aa) proprotein (aa 27‑181).  It contains a 132 aa mature region (aa 27-159) and a C-terminal prosegment that is cleaved to create a GPI linkage. The mature region possesses one
V‑type Ig-like domain (aa 27-122).  CD160 is found as a soluble, disulfide-linked 80 kDa multimer (likely trimer) that is generated by proteolysis of the GPI-linked form. This 80 kDa form, plus others, are highly resistant to reduction. There is also a 100-110 kDa multimeric transmembrane (TM) form that is associated with activated NK cells. It contains a 55 aa substitution for Gly180-Leu181, and shows a 20 aa TM segment between aa 163-182.  The TM form appears to have a splice variant that lacks aa 25-133.  Over aa 27-159, human CD160 shares 62% aa identity with mouse CD160.  

Entrez Gene IDs
11126 (Human); 54215 (Mouse); 502585 (Rat); 102117412 (Cynomolgus Monkey)
Alternate Names
BY55; BY55FLJ46513; CD160 antigenimmunoglobulin superfamily member; CD160 molecule; CD160; Natural killer cell receptor BY55; NK1; NK28

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Citation for Human CD160 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CD160 isoforms and regulation of CD4 and CD8 T-cell responses.
    Authors: El-Far, Mohamed, Pellerin, Charles, Pilote, Louise, Fortin, Jean-Fra, Lessard, Ivan A D, Peretz, Yoav, Wardrop, Elizabet, Salois, Patrick, Bethell, Richard, Cordingley, Michael, Kukolj, George
    J Transl Med, 2014;12(0):217.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CD160 Antibody

Average Rating: 4 (Based on 2 Reviews)

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Human CD160 Antibody
By Anonymous on 12/13/2019
Application: Microarray Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human CD160 Antibody
By Anonymous on 11/07/2018
Application: ELISA Sample Tested: Plasma,Serum Species: Human and Mouse