Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 528807
View all CD24 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD24
Ser27-Gly59 (predicted)
Accession # P25063

Specificity

Detects human CD24 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD24 Antibody

CD24 antibody in Human Colon by Immunohistochemistry (IHC-P).

CD24 in Human Colon.

CD24 was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Human CD24 Monoclonal Antibody (Catalog # MAB5248) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

CD24 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

CD24 in Human Ovarian Cancer Tissue.

CD24 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human CD24 Monoclonal Antibody (Catalog # MAB5248) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human CD24 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon and human ovarian cancer tissue

Reviewed Applications

Read 4 reviews rated 4 using MAB5248 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD24

CD24, also known as heat stable antigen (HSA), is a heavily and variably glycosylated 35‑60 kDa GPI-linked sialoprotein that is expressed on B cells, T cells, keratinocytes, and myofiber synaptic nuclei and is up‑regulated in a wide variety of cancers. It is also a marker for exosomes released into the urine and amniotic fluid. CD24 binds to P-Selectin on activated platelets and vascular endothelial cells. CD24 ligation induces tumor cell apoptosis and is important for the deletion of autoreactive T cells. Mature human CD24 shares 30% and 42% aa sequence identity with mouse and rat CD24, respectively.

Alternate Names

CD24, CD24A

Entrez Gene IDs

100133941 (Human); 12484 (Mouse)

Gene Symbol

CD24

UniProt

P25063

Additional CD24 Products

Product Documents for Human CD24 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD24 Antibody

For research use only

Citations for Human CD24 Antibody

Customer Reviews for Human CD24 Antibody (4)

4 out of 5
4 Customer Ratings
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human CD24 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Human CD24 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Melanoma tissue
    Species: Human
    Verified Customer | Posted 11/04/2020
    Human CD24 Antibody MAB5248
  • Human CD24 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human CD24 Antibody MAB5248
  • Human CD24 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/10/2018
  • Human CD24 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/09/2018

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies