Detects human CD27 Ligand/TNFSF7 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse CD27 Ligand, recombinant human (rh) CD30 Ligand, or rhCD40 Ligand is observed.
Monoclonal Mouse IgG2B Clone # 301731
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human CD27 Ligand/TNFSF7 Gln45-Pro193 Accession # AAA36175
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
CD27 Ligand/TNFSF7 in human PBMCs.
CD27 Ligand/TNFSF7 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD27 Ligand/TNFSF7 Monoclonal Antibody (Catalog # MAB2738) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD27 Ligand/TNFSF7
CD27 Ligand is a member of the tumor necrosis factor superfamily
(TNFSF7) and is also designated CD70. CD27 Ligand is a type II
transmembrane protein expressed by activated T and B cells. It binds to
CD27 on mature T cells and a subset of thymocytes. CD27 Ligand induces
the proliferation of costimulated T cells and enhances the generation
of cytolytic T cells.
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