Human CD300a/LMIR1 Antibody

R&D Systems | Catalog # AF2640

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA

Cited:

Bioassay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD300a/LMIR1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD300a/LMIR1
Leu18-Gln178
Accession # Q9UGN4

Specificity

Detects human CD300a/LMIR1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) LMIR2, rhLMIR3, rhLMIR5, and rhLMIR6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CD300a/LMIR1 Antibody

Detection of Human CD300a/LMIR1 antibody by Western Blot.

Detection of Human CD300a/LMIR1 by Western Blot.

Western blot shows lysates of human spleen tissue and human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD300a/LMIR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2640) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for CD300a/LMIR1 at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human CD300a/LMIR1 ELISA Standard Curve.

Recombinant Human CD300a/LMIR1 protein was serially diluted 2-fold and captured by Rat Anti-Human CD300a/LMIR1 Monoclonal Antibody (MAB26401) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CD300a/LMIR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2640) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human CD300a/LMIR1 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human CD300a/LMIR1 Monoclonal Antibody (Catalog # MAB26401).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human CD300a/LMIR1 DuoSet ELISA Kit (Catalog # DY2640-05) for convenient development of a sandwich ELISA.

Western Blot

1 µg/mL
Sample: Human spleen tissue and human granulocytes

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD300a/LMIR1

CD300a, also known as LMIR1 (in rodents), CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Human LMIR1 consists of a 163 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 98 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2). Alternate splicing may generate additional isoforms that either lack the Ig-like domain or contain only the cytoplasmic domain. Within the ECD, human LMIR1 shares 40% and 43% aa sequence identity with mouse and rat LMIR1, respectively. In human, LMIR1 is expressed on peripheral blood eosinophils, mast cells, neutrophils, plasmacytoid dendritic cells, and various T cell subsets (3‑7). Antibody crosslinking of LMIR1 induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5‑7). Crosslinking of LMIR1 to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (5, 10‑12). LMIR1 crosslinking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 11, 12).

References

  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148.
  3. Munitz, A. et al. (2006) Blood 107:1996.
  4. Bachelet, I. et al. (2005) J. Immunol. 175:7989.
  5. Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.
  6. Ju, X. et al. (2008) Blood 112:1184.
  7. Clark, G.J. et al. (2007) J. Leukoc. Biol. 82:1126.
  8. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.
  9. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
  10. Bachelet, I. et al. (2008) J. Immunol. 180:6064.
  11. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314.
  12. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.

Alternate Names

CD300a, CLM8, CMRF-35H, IGSF12, IRC1, IRC2, IRp60, LMIR1, MAIR-I, Mcipr1, NKRL, Pigr4

Entrez Gene IDs

11314 (Human); 217303 (Mouse)

Gene Symbol

CD300A

UniProt

Q9UGN4

Additional CD300a/LMIR1 Products

Product Documents for Human CD300a/LMIR1 Antibody

Certificate of Analysis

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Product Specific Notices for Human CD300a/LMIR1 Antibody

For research use only

Citations for Human CD300a/LMIR1 Antibody

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