Human CD3 epsilon Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon Recombinant Monoclonal Antibody (Catalog # MAB100, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody
(Catalog # F0102B).
CD3 epsilon in Human PBMCs. CD3 epsilon was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3 epsilon Recombinant Monoclonal Antibody (Catalog # MAB100R) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
T Cell Stimulation Induced by Human CD3 epsilon Antibody. Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100R) induces stimulation in human T cells in a dose-dependent manner, as measured by Resazurin (Catalog # AR002), when immobilized at 1-10 μg/mL (100 μL/well).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD3 epsilon
CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
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Cell and Tissue Staining Kits
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