Human CD3 epsilon Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB100R-100
MAB100R-SP
MAB100R-500
MAB100R-01M
Detection of Human CD3 epsilon  by Western Blot.
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Human CD3 epsilon Antibody Summary

Species Reactivity
Human
Specificity
Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
Source
Recombinant Monoclonal Mouse IgG1 Clone # UCHT1R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human thymocytes
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
MOLT‑4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line
Flow Cytometry
0.25 µg/106 cells
See below
Immunocytochemistry
1-25 µg/mL
See below
T Cell Stimulation
This antibody can be used to activate T cells when immobilized at 1-10 μg/mL (100 μL/well).
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human CD3 epsilon by Western Blot. Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line (negative control), and THP‑1 human acute monocytic leukemia cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD3 epsilon at approximately 21 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry Detection of CD3e antibody in Human Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e Recombinant Monoclonal Antibody (Catalog # MAB100, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Immunocytochemistry CD3e antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CD3 epsilon in Human PBMCs. CD3e was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3e Recombinant Monoclonal Antibody (Catalog # MAB100R) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; (NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

T Cell Stimulation T Cell Stimulation Induced by Human CD3 epsilon  Antibody. View Larger

T Cell Stimulation Induced by Human CD3 epsilon Antibody. Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100R) induces stimulation in human T cells in a dose-dependent manner, as measured by Resazurin (AR002), when immobilized at 1-10 µg/mL (100 µL/well).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

References
  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
Entrez Gene IDs
916 (Human); 12501 (Mouse)
Alternate Names
CD_antigen: CD3e; CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD18; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

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Human CD3 epsilon Antibody
By Anonymous on 12/02/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Cancer cell line,MOLT-3 cell line Species: Human

Human CD3 epsilon Antibody
By Anonymous on 02/16/2019
Application: Func Sample Tested: Peripheral blood T cells Species: Human

96-well plates were coated with MAB100R and incubated with isolated CD8+ T-cells from human PBMCs. Activation was read through intracellular interferon.