Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Immunocytochemistry, ELISA Capture, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 344519
View all CD46 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD46
Cys35-Asp328
Accession # NP_722548

Specificity

Detects human CD46 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CD46 Antibody

Detection of CD46 in PBMC lymphocytes by Flow Cytometry

PBMC lymphocytes were stained with Mouse Anti-Human CD46 Monoclonal Antibody (Catalog # MAB2005, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD46 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: see below

Western Blot

1 µg/mL
Sample: Recombinant Human CD46

Reviewed Applications

Read 1 review rated 5 using MAB2005 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD46

CD46, also known as MCP, is a type I membrane protein that functions as a cofactor for Complement Factor I-mediated inactivation of complement components C3b and C4b. CD46 is expressed in white blood cells, platelets, epithelial cells, and fibroblasts. The extracellular region of CD46 contains four tandem Sushi domains. At least fourteen transcript variants encode isoforms with deletions or substitutions following the fourth Sushi domain. Within corresponding regions, human CD46 shares 50% amino acid sequence identity with mouse and rat CD46.

Alternate Names

CD46, MCP

Entrez Gene IDs

4179 (Human); 17221 (Mouse)

Gene Symbol

CD46

UniProt

NP_722548

Additional CD46 Products

Product Documents for Human CD46 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD46 Antibody

For research use only

Citations for Human CD46 Antibody

Customer Reviews for Human CD46 Antibody (1)

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1 Customer Rating
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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 24163703
    Species: Human
    Verified Customer | Posted 02/16/2015

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