Human CD5 Antibody

Catalog # Availability Size / Price Qty
AF1636
AF1636-SP
Human CD5 Antibody in Western Blot
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Product Details
Citations (1)
FAQs
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Human CD5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD5 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD5
Arg25-Asn371
Accession # P06127
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
Human whole blood lymphocytes
Agonist Activity
2-8 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human CD5 by Western Blot. View Larger

Detection of Human CD5 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1636) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD5 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Agonist Activity Human CD5 Antibody Enhances IL-2 Secretion in Human T Cells. View Larger

Human CD5 Antibody Enhances IL-2 Secretion in Human T Cells. Human CD5 Antigen Affinity-purified Polyclonal Antibody enhances IL-2 secretion in human T cells in the presence of sub-optimal amounts of Human CD3 epsilon  Monoclonal Antibody (Catalog # MAB100) and Human CD28 Monoclonal Antibody (Catalog # MAB342), in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # D2050). The ED50 for this effect is typically 2‑8 μg/mL.

Immunocytochemistry CD5  in Human PBMCs. View Larger

CD5 in Human PBMCs. CD5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1636) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western Detection  of Human CD5 by Simple WesternTM. View Larger

Detection of Human CD5 by Simple WesternTM. Simple Western lane view shows lysates of CEM human
T-lymphoblastoid cell line, loaded at 0.2 mg/mL. A specific band was detected for CD5 at approximately 93 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1636). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD5

CD5, also known as Leu-1, is a type I transmembrane protein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily. It is expressed on thymocytes, T cells and the B1a subpopulation of B cells. CD5 binds the C-type lectin, CD72, and modulates signaling through the antigen receptors on T and B cells. CD5 has been shown to provide either positive or negative co-stimulatory signals depending on cell type to regulate immune responses.

References
  1. Ledbetter, J.A. et al. 1985, J. Immunol. 135:2331.
  2. Damle, N.K. et al. 1988, J. Immunol. 140:1753.
Entrez Gene IDs
921 (Human); 12507 (Mouse)
Alternate Names
CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1

Product Datasheets

Citation for Human CD5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia.
    Authors: Baskar S, Kwong KY, Hofer T, Levy JM, Kennedy MG, Lee E, Staudt LM, Wilson WH, Wiestner A, Rader C
    Clin. Cancer Res., 2008;14(2):396-404.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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