Detection of Human CD5 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1636) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD5 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Human CD5 Antibody Enhances IL-2 Secretion in Human T Cells.
Human CD5 Antigen Affinity-purified Polyclonal Antibody enhances IL-2 secretion in human T cells in the presence of sub-optimal amounts of Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) and Human CD28 Monoclonal Antibody (Catalog # MAB342), in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # D2050). The ED50 for this effect is typically 2‑8 μg/mL.
CD5 in Human PBMCs.
CD5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1636) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human CD5 by Simple WesternTM.
Simple Western lane view shows lysates of CEM human T-lymphoblastoid cell line, loaded at 0.2 mg/mL. A specific band was detected for CD5 at approximately 93 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1636). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD5, also known as Leu-1, is a type I transmembrane protein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily. It is expressed on thymocytes, T cells and the B1a subpopulation of B cells. CD5 binds the C-type lectin, CD72, and modulates signaling through the antigen receptors on T and B cells. CD5 has been shown to provide either positive or negative co-stimulatory signals depending on cell type to regulate immune responses.
Ledbetter, J.A. et al. 1985, J. Immunol. 135:2331.
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