Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 706931
View all CD79A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD79A
Leu33-Arg143
Accession # P11912

Specificity

Detects human CD79A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD79A is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD79A Antibody

Detection of CD79A antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of CD79A in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes treated with Recombinant Human IL-2 (202-IL) were stained with Mouse Anti-Human CD79A Monoclonal Antibody (Catalog # MAB69201) Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). Quadrant markers were set based on control antibody staining (MAB002).

Detection of CD79A in PBMC with CD19 costain cells by Flow Cytometry.

PBMC with CD19 costain were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CD79A Monoclonal Antibody (Catalog # MAB69201) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD79A Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood monocytes treated with Recombinant Human IL-2 (Catalog # 202-IL) and PBMC with CD19 costain

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD79A

CD79A, also known as Ig alpha and MB-1, is a 44 kDa transmembrane glycoprotein in the immunoglobulin superfamily. It contains a single Ig-like domain in its extracellular region (ECD) and one cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM). Alternate splicing generates a short isoform with a 39 aa deletion in the ECD. Heterodimers of CD79A and CD79B/Ig beta associate with a membrane bound immunoglobulin on the B cell surface to form the B cell antigen receptor complex (BCR). CD79A and CD79B are required for BCR-mediated signaling and consequently for the development and activation of B lineage cells. Within the ECD, human CD79A shares 57% aa sequence identity with mouse and rat CD79A.

Alternate Names

CD79A, MB-1

Entrez Gene IDs

973 (Human); 12518 (Mouse); 681236 (Rat); 722190 (Rhesus Macaque)

Gene Symbol

CD79A

UniProt

P11912

Additional CD79A Products

Product Documents for Human CD79A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD79A Antibody

For research use only

Related Research Areas

B Cell Activation

Citations for Human CD79A Antibody

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