Human CDNF Antibody

Catalog # Availability Size / Price Qty
CDNF in Human Brain.
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Product Details
Citations (2)
Supplemental Products

Human CDNF Antibody Summary

Species Reactivity
Detects human CDNF in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant mouse CDNF is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human CDNF
Accession # Q49AH0
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human CDNF (Catalog # 5097-CD)
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CDNF antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

CDNF in Human Brain. CDNF was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 10 µg/mL Goat Anti-Human CDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5097) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDNF

CDNF (conserved dopamine neurotrophic factor), also called Armetl1 (arginine-rich, mutated in early stage tumors-like 1) in mouse, is a 17-19 kDa secreted protein that shares 62% amino acid (aa) identity with human MANF (mesencephalic-astrocyte-derived neurotrophic factor), also called Armet in mouse (1). The Armet designation is not preferred, because the proteins when translated are not actually arginine-rich (1). However, both CDNF and MANF have a high proportion of charged residues, a pattern of eight cysteines shown to form intramoleculular disulfides, and a C-terminal endoplasmic reticulum retention signal (shown to function in MANF) (1-3). The human CDNF cDNA encodes a 187 aa protein with a 24 aa signal sequence and a 163 mature sequence (1). Mature human CDNF shares 80%, 84%, 90%, and 92% aa identity with mouse, rat, equine, and bovine CDNF, respectively. Although CDNF mRNA and protein are expressed in pre- and post-natal mouse brain, they are most abundant in adult heart, skeletal muscle and testis. Transcripts within the postnatal mouse brain are concentrated in the hippocampus, thalamus, corpus callosum and optic nerve (1). Like MANF and GDNF, CDNF promotes survival of dopaminergic neurons in vitro (1, 4). In a rat Parkinson’s disease model, CDNF also promotes rescue and restoration of dopaminergic neurons in vivo (1).

  1. Lindholm, P. et al. (2007) Nature 448:73.
  2. Mizobuchi, N. et al. (2007) Cell Struct. Funct. 32:41.
  3. Raykhel, I. et al. (2007) J. Cell Biol. 179:1193.
  4. Petrova, P. et al. (2003) J. Mol. Neurosci. 20:173.
Long Name
Conserved Dopamine Neurotrophic Factor
Entrez Gene IDs
441549 (Human); 227526 (Mouse); 361276 (Rat)
Alternate Names
arginine-rich, mutated in early stage tumors-like 1; ARMETL1; ARMET-like protein 1; CDNF; cerebral dopamine neurotrophic factor; Conserved dopamine neurotrophic factor

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Citations for Human CDNF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Human-Specific Regulation of Neurotrophic Factors MANF and CDNF by microRNAs
    Authors: J Konovalova, D Gerasymchu, SN Arroyo, S Kluske, F Mastroiann, AV Pereyra, A Domanskyi
    International Journal of Molecular Sciences, 2021;22(18):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  2. Increased Serum Levels of Mesencephalic Astrocyte-Derived Neurotrophic Factor in Subjects With Parkinson's Disease
    Authors: E Galli, A Planken, L Kadastik-E, M Saarma, P Taba, P Lindholm
    Front Neurosci, 2019;13(0):929.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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