Human CDNF Antibody Summary
Accession # Q49AH0
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CDNF in Human Brain. CDNF was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 10 µg/mL Goat Anti-Human CDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5097) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CDNF (conserved dopamine neurotrophic factor), also called Armetl1 (arginine-rich, mutated in early stage tumors-like 1) in mouse, is a 17-19 kDa secreted protein that shares 62% amino acid (aa) identity with human MANF (mesencephalic-astrocyte-derived neurotrophic factor), also called Armet in mouse (1). The Armet designation is not preferred, because the proteins when translated are not actually arginine-rich (1). However, both CDNF and MANF have a high proportion of charged residues, a pattern of eight cysteines shown to form intramoleculular disulfides, and a C-terminal endoplasmic reticulum retention signal (shown to function in MANF) (1-3). The human CDNF cDNA encodes a 187 aa protein with a 24 aa signal sequence and a 163 mature sequence (1). Mature human CDNF shares 80%, 84%, 90%, and 92% aa identity with mouse, rat, equine, and bovine CDNF, respectively. Although CDNF mRNA and protein are expressed in pre- and post-natal mouse brain, they are most abundant in adult heart, skeletal muscle and testis. Transcripts within the postnatal mouse brain are concentrated in the hippocampus, thalamus, corpus callosum and optic nerve (1). Like MANF and GDNF, CDNF promotes survival of dopaminergic neurons in vitro (1, 4). In a rat Parkinson’s disease model, CDNF also promotes rescue and restoration of dopaminergic neurons in vivo (1).
- Lindholm, P. et al. (2007) Nature 448:73.
- Mizobuchi, N. et al. (2007) Cell Struct. Funct. 32:41.
- Raykhel, I. et al. (2007) J. Cell Biol. 179:1193.
- Petrova, P. et al. (2003) J. Mol. Neurosci. 20:173.
Citation for Human CDNF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Increased Serum Levels of Mesencephalic Astrocyte-Derived Neurotrophic Factor in Subjects With Parkinson's Disease
Authors: E Galli, A Planken, L Kadastik-E, M Saarma, P Taba, P Lindholm
Front Neurosci, 2019;13(0):929.
Sample Types: Serum
Applications: ELISA Capture
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Cell and Tissue Staining Kits
Reviews for Human CDNF Antibody
There are currently no reviews for this product. Be the first to review Human CDNF Antibody and earn rewards!
Have you used Human CDNF Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image