Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Bioassay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CDX2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CDX2
Asn54-Thr185
Accession # Q99626

Specificity

Detects human CDX2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human CDX2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to trophectoderm using Mouse Embryonic Fibroblast (MEF) Conditioned Media (Catalog # AR005) with 10 ng/mL recombinant human FGF-basic (Catalog # 233-FB-025) and 100 ng/mL recombinant human BMP-4 (Catalog # 314-BP-010) for 7 days

Western Blot

0.1 µg/mL
Sample: Recombinant Human CDX2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDX2

Human CDX2 (Caudal type homeobox transcription factor 2) is a 37 kDa member of the caudal family of homeobox proteins. It is 313 amino acids (aa) in length and contains a 167 aa caudal-like activation domain (aa 13‑180), a DNA-binding homeodomain (aa 186‑245) and two short, poly-glutamine and -proline repeats. CDX2 can exist as a dimer and is regulated by phosphorylation of a 4(x)serine motif that promotes CDX2 degradation. CDX2 promotes intestinal patterning and development. While it is necessary but not sufficient for intestinal phenotype differentiation, it will block cell proliferation on its own. Over the amino acid range used for immunization, human CDX2 is 95% and 97% aa identical to mouse and canine CDX2, respectively.

Long Name

Caudal-type Homeobox Transcription Factor 2

Alternate Names

CDX3

Entrez Gene IDs

1045 (Human); 12591 (Mouse); 66019 (Rat)

Gene Symbol

CDX2

UniProt

Q99626

Additional CDX2 Products

Product Documents for Human CDX2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CDX2 Antibody

For research use only

Citations for Human CDX2 Antibody

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Protocols

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FAQs

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