Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 913542
View all CEACAM-8/CD66b Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human CEACAM-8/CD66b
Gln35-His141
Accession # P31997

Specificity

Detects human CEACAM-8/CD66b in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CEACAM‑8/CD66b Antibody

Detection of CEACAM-8 in Human Colon Cancer via seqIF™ staining on COMET™

CEACAM-8 was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human CEACAM-8, Monoclonal Antibody (Catalog #MAB4246) at 1ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​

Detection of CEACAM-8 in Human Hodgkin Lymphoma via seqIF™ staining on COMET™

CEACAM-8 was detected in immersion fixed paraffin-embedded sections of human Hodgkin Lymphoma using Mouse Anti-Human CEACAM-8, Monoclonal Antibody (Catalog #MAB4246) at 1ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​

Detection of CEACAM-8 in Human Tonsil via seqIF™ staining on COMET™

CEACAM-8 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CEACAM-8, Monoclonal Antibody (Catalog #MAB4246) at 1ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
Detection of Human CEACAM-8/CD66b antibody by Western Blot.

Detection of Human CEACAM‑8/CD66b by Western Blot.

Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) and human peripheral blood granulocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CEACAM-8/CD66b Monoclonal Antibody (Catalog # MAB4246) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CEACAM-8/CD66b at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CEACAM-8/CD66b antibody in Human Blood Granulocytes antibody by Flow Cytometry.

Detection of CEACAM‑8/CD66b in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human CEACAM-8/CD66b Monoclonal Antibody (Catalog # MAB4246, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Detection of CEACAM‑8/CD66b in Human Spleen.

CEACAM‑8/CD66b was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CEACAM‑8/CD66b Monoclonal Antibody (Catalog # MAB4246) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human CEACAM‑8/CD66b Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood granulocytes

Immunohistochemistry

0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Western Blot

2 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMC) and human peripheral blood granulocytes

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-8/CD66b

CEACAM-8 (Carcinoembryonic Antigen-related Cell Adhesion Molecule 8), also known as CD66b, CD67 and NCA-95, is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human
CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Alternate Names

CD66b, CD67, CEACAM8, CGM6, NCA-95

Entrez Gene IDs

1088 (Human); 102138843 (Cynomolgus Monkey)

Gene Symbol

CEACAM8

UniProt

P31997

Additional CEACAM-8/CD66b Products

Product Documents for Human CEACAM‑8/CD66b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CEACAM‑8/CD66b Antibody

For research use only

Citations for Human CEACAM‑8/CD66b Antibody

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Protocols

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