Human Cerebellin-2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Cerebellin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cerebellin-1, -3, or -4 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 704330
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
     Chinese hamster ovary cell line CHO-derived recombinant human Cerebellin-2
    Arg50-Leu224
    Accession # Q8IUK8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse Cerebellin-2 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human brain (cortex) tissue, human brain (hippocampus) tissue, human brain (hypothalamus) tissue, and mouse brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cerebellin-2 Monoclonal Antibody (Catalog # MAB7044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cerebellin-2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cerebellin-2
Cerebellin-2 (CBLN2) is an approximately 25 kDa glycoprotein in the Cerebellin family of C1q- and TNF-related proteins. It contains a potential membrane-associating segment but may also be secreted. It contains one C1q-like domain in its extracellular region. Cerebellin-2 expression is developmentally regulated in the cerebellum, neocortex, sensory neurons of the dorsal root ganglion and spinal cord, and adrenal cortex. It associates with Cerebellin-1 which is involved in the formation and maintenance of synapses in the cerebellum. Within the ectodomain (aa 50-224), human Cerebellin-2 shares 99% aa sequence identity with mouse and rat Cerebellin-2.
  • Entrez Gene IDs:
    147381 (Human); 12405 (Mouse); 291388 (Rat)
  • Alternate Names:
    CBLN2; cerebellin 2 precursor; Cerebellin2; Cerebellin-2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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