Detection of Human and Mouse Cerebellin-2 by Western Blot.
Western blot shows lysates of human brain (cerebellum) tissue, human brain (cortex) tissue, human brain (hippocampus) tissue, human brain (hypothalamus) tissue, and mouse brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cerebellin-2 Monoclonal Antibody (Catalog # MAB7044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cerebellin-2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cerebellin-2 (CBLN2) is an approximately 25 kDa glycoprotein in the Cerebellin family of C1q- and TNF-related proteins. It contains a potential membrane-associating segment but may also be secreted. It contains one C1q-like domain in its extracellular region. Cerebellin-2 expression is developmentally regulated in the cerebellum, neocortex, sensory neurons of the dorsal root ganglion and spinal cord, and adrenal cortex. It associates with Cerebellin-1 which is involved in the formation and maintenance of synapses in the cerebellum. Within the ectodomain (aa 50-224), human Cerebellin-2 shares 99% aa sequence identity with mouse and rat Cerebellin-2.
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