Detection of Human and Mouse CHD1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, Raji human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line. PVDF Membrane was probed with 0.5 µg/mL of Human CHD1 Monoclonal Antibody (Catalog # MAB6195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CHD1 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CHD1 in BG01V Human Stem Cells. CHD1 was detected in immersion fixed BG01V human embryonic stem cells using Human CHD1 Monoclonal Antibody (Catalog # MAB6195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CHD1 (Chromohelicase/ATPase DNA-binding protein 1) is a 200-205 kDa member of the SNF2/RAD54 helicase family of proteins. It is an ATP-dependent chromatin remodeling factor that helps maintain chromatin in a transcriptionally active state. In embryonic stem cells, CHD1 associates with the promoters of active genes, a condition that is associated with open chromatin and pluripotency. Human CHD1 is 1710 amino acids (aa) in length. It contains two Ser-rich regions (aa 1-70 and 117‑137), two Chromo (chromatin-organizer-modifer) domains (aa 272-364 and 389-452), a SNF2 family N-terminal domain (aa 484-763) and a C-terminal helicase domain (aa 792-943). There are at least 30 Ser/Thr phosphorylation sites. Over aa 1531-1710, human CHD1 shares 92% aa identity with mouse CHD1.
Chromodomain Helicase DNA Binding Protein 1
Entrez Gene IDs:
1105 (Human); 12648 (Mouse)
ATP-dependent helicase CHD1; CHD-1; chromodomain helicase DNA binding protein 1; chromodomain-helicase-DNA-binding protein 1; DKFZp686E2337; EC 3.6.1; EC 188.8.131.52
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