Detection of Human CHIP/STUB1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CHIP/STUB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4685) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CHIP/STUB1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
CHIP/STUB1 in HeLa Human Cell Line.
CHIP/STUB1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human CHIP/STUB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4685) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
STUB1 (STIP1 homology and U-box-containing protein 1, also known as C-terminus of Hsc70-interacting protein, CHIP) is a 35 kDa ubiquitin ligase that participates in intracellular protein folding/refolding and degradation. It is highly expressed in striated muscle. STUB1/CHIP may complex with the molecular chaperones Hsp70 and Hsp90, or act by itself. In either event, it regulates protein stability and ubiquitination. Human STUB1/CHIP is 303 amino acids (aa) in length. It contains one TPR domain (aa 27‑127) plus a C-terminal U-Box (aa 226‑299). Its TRP domain interacts with heat shock proteins, while the U-Box participates in ubiquitination. There is one isoform that shows an alternate start site at Met73. Human and mouse STUB1 are 97% aa identical.
C-terminus of Hsc70 Interacting Protein
Entrez Gene IDs:
10273 (Human); 56424 (Mouse)
Antigen NY-CO-7; Carboxy terminus of Hsp70-interacting protein; CHIP; CHIPSTIP1 homology and U box-containing protein 1; CLL-associated antigen KW-8; E3 ubiquitin-protein ligase CHIP; EC 6.3.2.-; heat shock protein A binding protein 2 (c-terminal); HSPABP2; NY-CO-7; SDCCAG7; serologically defined colon cancer antigen 7; STIP1 homology and U-box containing protein 1; STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase; STUB1; UBOX1
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