Human CHIP/STUB1 Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human CHIP/STUB1 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CHIP/STUB1
    Met1-Tyr303
    Accession # Q9UNE7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CHIP/STUB1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CHIP/STUB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4685) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CHIP/STUB1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunocytochemistry
CHIP/STUB1 in HeLa Human Cell Line. CHIP/STUB1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human CHIP/STUB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4685) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CHIP/STUB1

STUB1 (STIP1 homology and U-box-containing protein 1, also known as C-terminus of Hsc70-interacting protein, CHIP) is a 35 kDa ubiquitin ligase that participates in intracellular protein folding/refolding and degradation. It is highly expressed in striated muscle. STUB1/CHIP may complex with the molecular chaperones Hsp70 and Hsp90, or act by itself. In either event, it regulates protein stability and ubiquitination. Human STUB1/CHIP is 303 amino acids (aa) in length. It contains one TPR domain (aa 27‑127) plus a C-terminal U-Box (aa 226‑299). Its TRP domain interacts with heat shock proteins, while the U-Box participates in ubiquitination. There is one isoform that shows an alternate start site at Met73. Human and mouse STUB1 are 97% aa identical.

  • Long Name:
    C-terminus of Hsc70 Interacting Protein
  • Entrez Gene IDs:
    10273 (Human); 56424 (Mouse)
  • Alternate Names:
    Antigen NY-CO-7; Carboxy terminus of Hsp70-interacting protein; CHIP; CHIPSTIP1 homology and U box-containing protein 1; CLL-associated antigen KW-8; E3 ubiquitin-protein ligase CHIP; EC 6.3.2.-; heat shock protein A binding protein 2 (c-terminal); HSPABP2; NY-CO-7; SDCCAG7; serologically defined colon cancer antigen 7; STIP1 homology and U-box containing protein 1; STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase; STUB1; UBOX1
Related Research Areas

FAQs

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