Detection of Human CHIP/STUB1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CHIP/STUB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4685) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CHIP/STUB1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
STUB1 (STIP1 homology and U-box-containing protein 1, also known as C-terminus of Hsc70-interacting protein, CHIP) is a 35 kDa ubiquitin ligase that participates in intracellular protein folding/refolding and degradation. It is highly expressed in striated muscle. STUB1/CHIP may complex with the molecular chaperones Hsp70 and Hsp90, or act by itself. In either event, it regulates protein stability and ubiquitination. Human STUB1/CHIP is 303 amino acids (aa) in length. It contains one TPR domain (aa 27‑127) plus a C-terminal U-Box (aa 226‑299). Its TRP domain interacts with heat shock proteins, while the U-Box participates in ubiquitination. There is one isoform that shows an alternate start site at Met73. Human and mouse STUB1 are 97% aa identical.
C-terminus of Hsc70 Interacting Protein
Entrez Gene IDs:
10273 (Human); 56424 (Mouse)
Antigen NY-CO-7; Carboxy terminus of Hsp70-interacting protein; CHIP; CHIPSTIP1 homology and U box-containing protein 1; CLL-associated antigen KW-8; E3 ubiquitin-protein ligase CHIP; EC 6.3.2.-; heat shock protein A binding protein 2 (c-terminal); HSPABP2; NY-CO-7; SDCCAG7; serologically defined colon cancer antigen 7; STIP1 homology and U-box containing protein 1; STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase; STUB1; UBOX1
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.