Detection of Human CIB1 by Western Blot.
Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CIB1 Monoclonal Antibody (Catalog # MAB7557) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CIB1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CIB1 in Human Breast Cancer Tissue.
CIB1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human CIB1 Monoclonal Antibody (Catalog # MAB7557) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calcium and Integrin binding protein 1 (CIB1), also known as Calmyrin, is a ubiquitously expressed 22 kDa myristoylated and membrane-associated protein. It contains two EF hand domains (aa 103-138 and aa 148-183). Calmyrin interacts with a range of proteins including Integrin alpha 2b and GPIIb/IIIa, Presenilin-2, InsP3R, Stathmin-2, Myosin 1C, Sphingosine Kinase 1, Polo-like kinases, and Rac3. Human Calmyrin shares 94% aa sequence identity with mouse and rat Calmyrin.
Calcium and Integrin Binding 1
Entrez Gene IDs:
10519 (Human); 23991 (Mouse); 81823 (Rat)
calcium and integrin binding 1 (calmyrin); Calcium- and integrin-binding protein; Calmyrin; CIB1; CIBcalcium and integrin binding protein; CIBP; DNA-dependent protein kinase interacting protein; DNA-PKcs-interacting protein; Kinase-interacting protein; KIP; KIP1; KIPcalcium and integrin binding, protein kinase interacting protein; PRKDCIP; SIP2-28; SIP2-28calcium and integrin-binding protein 1; Snk interacting protein 2-28; SNK-interacting protein 2-28
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