Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Flow Cytometry, Immunoprecipitation, Bioassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 138815
View all CLC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CLC
Leu28-Phe225
Accession # Q9UBD9

Specificity

Detects human CLC in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CT-1, rhCNTF, rhIL-6, rhIL-11, rhLIF, or rhOSM is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CLC Antibody

CLC antibody in Human Prostate by Immunohistochemistry (IHC-P).

CLC in Human Prostate.

CLC was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human CLC Monoclonal Antibody (Catalog # MAB962) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human CLC Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Western Blot

1 µg/mL
Sample: Recombinant Human CLC (Catalog # 962-CL)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CLC

Cardiotrophin-like cytokine (CLC) (also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3)) is a recently discovered 22-25 kDa member of the IL-6 family of cytokines (1, 2, 3). As such, it is expressed as a long type I cytokine with four alpha -helices in it structure (2). Human CLC is synthesized as a 225 amino acid precursor that contains a 27 aa signal sequence and a 198 aa mature region. It contains one potential N-linked glycosylation site that is apparently utilized, and two distinct binding sites for CNTFR alpha and CLF (4, 5). Although CLC has a signal sequence, it is not secreted unless noncovalently dimerized to either CLF or soluble CNTFR alpha (5, 6). Once dimerized, CLC signals through a tripartite receptor complex composed of gp130, LIFR beta and CNTFR alpha (membrane-bound) (5, 7). Within the IL-6 family, human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence identity (8).  Human to mouse, mature CLC is 96% aa identical. CLC is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation (9, 10, 11). Cells known to express CLC include embryonic muscle, lung epithelium, and mesenchyme in various regions (12).

References

  1. Murakami, M. et al. (2004) Growth Factors 22:75.
  2. Ernst, M. & B.J. Jenkins (2004) Trends Genet. 20:23.
  3. Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
  4. Perret, D. et al. (2003) J. Biol. Chem. 279:43961.
  5. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
  6. Elson, G.C.A. et al. (2000) Nat. Neurosci. 3:867.
  7. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
  8. Derouet, D. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4827.
  9. Forger, N.G. et al. (2003) J. Neurosci. 23:8854.
  10. Auernhammer, C.J. et al. (2003) Endocrinology 144:1202.
  11. Senaldi, G. et al. (2002) J. Immunol. 168:5690.
  12. de Bovis, B. et al. (2005) Cell Commun. Signal. 3:1.

Long Name

Cardiotrophin-like Cytokine

Alternate Names

BSF-3, CISS2, CLCF1, NNT-1, NR6

Entrez Gene IDs

23529 (Human); 56708 (Mouse); 365395 (Rat)

Gene Symbol

CLCF1

UniProt

Q9UBD9

Additional CLC Products

Product Documents for Human CLC Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CLC Antibody

For research use only

Related Research Areas

Diabetes
IL-6 Family

Citations for Human CLC Antibody

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Protocols

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