CLC was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human CLC Monoclonal Antibody (Catalog # MAB962) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cardiotrophin-like cytokine (CLC) (also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3)) is a recently discovered 22-25 kDa member of the IL-6 family of cytokines (1, 2, 3). As such, it is expressed as a long type I cytokine with four alpha -helices in it structure (2). Human CLC is synthesized as a 225 amino acid precursor that contains a 27 aa signal sequence and a 198 aa mature region. It contains one potential N-linked glycosylation site that is apparently utilized, and two distinct binding sites for CNTFR alpha and CLF (4, 5). Although CLC has a signal sequence, it is not secreted unless noncovalently dimerized to either CLF or soluble CNTFR alpha (5, 6). Once dimerized, CLC signals through a tripartite receptor complex composed of gp130, LIFR beta and CNTFR alpha (membrane-bound) (5, 7). Within the IL-6 family, human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence identity (8). Human to mouse, mature CLC is 96% aa identical. CLC is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation (9, 10, 11). Cells known to express CLC include embryonic muscle, lung epithelium, and mesenchyme in various regions (12).
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Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
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Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
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Forger, N.G. et al. (2003) J. Neurosci. 23:8854.
Auernhammer, C.J. et al. (2003) Endocrinology 144:1202.
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de Bovis, B. et al. (2005) Cell Commun. Signal. 3:1.
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