Human CLC Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CLC in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CT-1, rhCNTF, rhIL-6, rhIL-11, rhLIF, or rhOSM is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 138815
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human CLC
    Leu28-Phe225
    Accession # Q9UBD9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CLC (Catalog # 962-CL)
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

CLC in Human Prostate. CLC was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human CLC Monoclonal Antibody (Catalog # MAB962) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLC
Cardiotrophin-like cytokine (CLC) (also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3)) is a recently discovered 22-25 kDa member of the IL-6 family of cytokines (1, 2, 3). As such, it is expressed as a long type I cytokine with four alpha -helices in it structure (2). Human CLC is synthesized as a 225 amino acid precursor that contains a 27 aa signal sequence and a 198 aa mature region. It contains one potential N-linked glycosylation site that is apparently utilized, and two distinct binding sites for CNTFR alpha and CLF (4, 5). Although CLC has a signal sequence, it is not secreted unless noncovalently dimerized to either CLF or soluble CNTFR alpha (5, 6). Once dimerized, CLC signals through a tripartite receptor complex composed of gp130, LIFR beta and CNTFR alpha (membrane-bound) (5, 7). Within the IL-6 family, human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence identity (8).  Human to mouse, mature CLC is 96% aa identical. CLC is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation (9, 10, 11). Cells known to express CLC include embryonic muscle, lung epithelium, and mesenchyme in various regions (12).
  • References:
    1. Murakami, M. et al. (2004) Growth Factors 22:75.
    2. Ernst, M. & B.J. Jenkins (2004) Trends Genet. 20:23.
    3. Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
    4. Perret, D. et al. (2003) J. Biol. Chem. 279:43961.
    5. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
    6. Elson, G.C.A. et al. (2000) Nat. Neurosci. 3:867.
    7. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
    8. Derouet, D. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4827.
    9. Forger, N.G. et al. (2003) J. Neurosci. 23:8854.
    10. Auernhammer, C.J. et al. (2003) Endocrinology 144:1202.
    11. Senaldi, G. et al. (2002) J. Immunol. 168:5690.
    12. de Bovis, B. et al. (2005) Cell Commun. Signal. 3:1.
  • Long Name:
    Cardiotrophin-like Cytokine
  • Entrez Gene IDs:
    23529 (Human); 56708 (Mouse); 365395 (Rat)
  • Alternate Names:
    B-cell stimulating factor 3; B-cell stimulatory factor 3; BSF-3; BSF3BSF-3; cardiotrophin-like cytokine factor 1; CISS2; CLC; CLCF1; CLCNNT-1; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; NNT-1; NNT1B-cell-stimulating factor 3; Novel neurotrophin-1; NR6
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