Human CLCA1 Antibody Summary
Accession # A8K714
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CCL1/I‑309/TCA‑3 in Human Colon. CCL1/I‑309/TCA‑3 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CCL1/I‑309/TCA‑3 Monoclonal Antibody (Catalog # MAB10766) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Goblet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human CLCA1 by Simple WesternTM. Simple Western lane view shows lysates of human small intestine, loaded at 0.2 mg/mL. Specific bands were detected for CLCA1 at approximately 55 and 89 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human CLCA1 Monoclonal Antibody (Catalog # MAB10766). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CLCA1 is a member of the calcium sensitive chloride conductance protein family which may be involved in mediating calcium-activated chloride conductance. This protein is expressed in goblet cells and intestinal basal crypt epithelial of the small intestine and colon. CLCA1 regulates mucin expression and participates in innate immune responses by binding to yet unidentified molecules on inflammatory cells for cytokine and chemokine production. CLCA1 also targets lymphatic endothelial cells and cancer cells. CLCA1 is a candidate as a modulating factor in the pathogenesis of mucus hypersecretory-associated respiratory and gastrointestinal diseases including asthma, COPD, cystic fibrosis, pneumonia, colon colitis, cystic fibrosis intestinal mucous disease, ulcerative colitis, and gastrointestinal parasitic infection.
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