Human CLCA1 Antibody

CCL1/I‑309/TCA‑3 in Human Colon.

CCL1/I‑309/TCA‑3 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CCL1/I‑309/TCA‑3 Monoclonal Antibody (Catalog # MAB10766) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Goblet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human CLCA1 by Simple Western^TM.

Simple Western lane view shows lysates of human small intestine, loaded at 0.2 mg/mL. Specific bands were detected for CLCA1 at approximately 55 and 89 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human CLCA1 Monoclonal Antibody (Catalog # MAB10766). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.