Human CLP24 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CLP24 in ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 652424
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CLP24
    Accession # Q9BSN7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CLP24 in CLP24 and eGFP Co-Transfected Cells by Flow Cytometry. Human CLP24 and eGFP co-transfectants were stained with Mouse Anti-Human CLP24 Monoclonal Antibody (Catalog # MAB6747) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
Immunocytochemistry
CLP24 in NTera‑2 Human Cell Line. CLP24 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Mouse Anti-Human CLP24 Monoclonal Antibody (Catalog # MAB6747) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLP24
TMEM204, also known as Claudin-like protein of 24 kDa (CLP24), is a cell surface protein with four transmembrane domains and a C-terminal PDZ motif. TMEM204 localizes to adherens junctions between epithelial and endothelial cells. It is involved in regulating paracellular flux and is upregulated under hypoxic conditions. Human TMEM204 shares 88% aa sequence identity with mouse and rat TMEM204.
  • Long Name:
    Claudin-like Protein 24
  • Entrez Gene IDs:
    79652 (Human); 407831 (Mouse); 287129 (Rat)
  • Alternate Names:
    C16orf30; C16orf30chromosome 16 open reading frame 30; Claudin-like protein 24; CLP24; CLP24MGC111564; FLJ20898; TMEM204; transmembrane protein 204
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