Human Coagulation Factor XI Light Chain Antibody

Catalog # Availability Size / Price Qty
MAB24601
MAB24601-SP
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Human Coagulation Factor XI Light Chain Antibody Summary

Species Reactivity
Human
Specificity
Detects human Coagulation Factor XI Light Chain in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) UPA-1, rhThrombin, rhKLK1, rhKLK3, rhKLK4, rhKLK5, rhKLK6, rhKLK8, rhKLK11, rhHGFA, rhFactor VII, or rhFactor X is observed. This antibody recognizes mature chain (aa 19‑625), light chain (aa 388‑625) and the active enzyme, which contains disulfide bond-linked light and heavy chains.
Source
Monoclonal Mouse IgG2B Clone # 318104
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor XI
Glu19-Val625
Accession # P03951
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Coagulation Factor XI (Catalog # 2460-SE)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Coagulation Factor XI (Catalog # 2460-SE), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human Coagulation Factor XI (2 µg/mL, Catalog # 2460-SE) cleavage of the fluorogenic peptide substrate Boc-IEGR-Amc(100 µM). The Neutralization Dose (ND50) is typically 2.5 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor XI

Coagulation factors XI and XIa refer to the pro and active forms of the same protease, respectively (1). Factor XI is synthesized in the liver and circulates in the plasma as a disulfide bond-linked dimer complexed with high molecular weight kininogen. Factor XI is converted into XIa via either the contact phase of blood coagulation or thrombin-mediated activation on the platelet surface. The resulting XIa converts factor IX into IXa, which subsequently activates factor X into Xa. Factor Xa in turn activates factor II/thrombin to complete the coagulation cascade. Patients with factor XI deficiency are prone to excessive bleeding after hemostatic challenge. There are two alternative splicing forms. Isoform 1 corresponds to the circulating plasma factor XI and isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells (2). The 625 amino acid (aa) precursor of isoform 1 consists of a signal peptide (aa 1-18) and the mature chain (aa 19-625). The mature chain (XI) can be further processed into the heavy chain (aa 19-387) and the light chain (aa 388-625) (XIa).

References
  1. Wash, P.N. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1651.
  2. Hsu, T.C. et al. (1998) J. Biol. Chem. 273:13787.
Entrez Gene IDs
2160 (Human); 109821 (Mouse)
Alternate Names
Coagulation Factor XI; EC 3.4.21; EC 3.4.21.27; FXIPlasma thromboplastin antecedent; MGC141891; PTA

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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