Chicken ovalbumin upstream promoter transcription factor I (COUP-TF I, EAR3, COUP-TFA; NR2F1) is a member of the orphan nuclear receptor family. COUP-TF I is expressed in specific regions of the rostral brain. COUP-TF I has varied roles in the development of the peripheral nervous system, such as early regionalization of the neocortex, differentiation of subplate neurons and guidance of thalamocortical axons. COUP-TFs have been shown to interact with a number of other nuclear receptors.
Human COUP-TF I/NR2F1 Antibody (Clone H8132)
R&D Systems | Catalog # PP-H8132-00
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat, Primate - Pan troglodytes (Chimpanzee)
Applications
Validated:
Immunohistochemistry, Western Blot, Immunoprecipitation
Cited:
Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # H8132
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Product Specifications
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human COUP-TF I/NR2F1 Antibody (Clone H8132)
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate
Sample: Immersion fixed paraffin-embedded sections of human prostate
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4.3 using PP-H8132-00 in the following applications:
Formulation, Preparation, and Storage
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: COUP-TF I/NR2F1
Long Name
COUP Transcription Factor 1/Nuclear Receptor Subfamily 2, Group F, Member 1
Alternate Names
COUPTF I, ERBAL3, NR2F1
Entrez Gene IDs
7025 (Human)
Gene Symbol
NR2F1
Additional COUP-TF I/NR2F1 Products
Product Documents for Human COUP-TF I/NR2F1 Antibody (Clone H8132)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human COUP-TF I/NR2F1 Antibody (Clone H8132)
For research use only
Related Research Areas
Citations for Human COUP-TF I/NR2F1 Antibody (Clone H8132)
Customer Reviews for Human COUP-TF I/NR2F1 Antibody (Clone H8132) (4)
4.3 out of 5
4 Customer Ratings
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Application: ImmunohistochemistrySample Tested: Adult testisSpecies: HumanVerified Customer | Posted 11/21/2024Nr2F1 (pink), IHC-P, 1:100
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Application: ImmunocytochemistrySample Tested: IGR39 human melanoma cell lineSpecies: HumanVerified Customer | Posted 11/16/2023Melanoma cell line stained with 1:100 dilution of PP-H8132-00 (red) after PFA fixationBio-Techne ResponseThis review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
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Application: Western BlotSample Tested: Brain tissueSpecies: HumanVerified Customer | Posted 09/21/2021
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Application: ImmunohistochemistrySample Tested: Optic vesicleSpecies: HumanVerified Customer | Posted 07/27/2021
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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