Human CRHBP Antibody

Name: Human CRHBP Antibody
Brand: R&D Systems
SKU: AF2796-SP
Rating: 3.8 (4 reviews)

Catalog #: AF2796
2 Citations 4 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2796
AF2796-SP

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Product Details

Citations (2)

FAQs

Supplemental Products

Reviews (4)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CRHBP Antibody Summary

Species Reactivity

Human

Specificity

Detects human CRHBP in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse CRHBP is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CRHBP
Tyr25-Leu322
Accession # P24387.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CRHBP

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human liver

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRHBP

Human CRHBP is a 37 kDa secreted glycoprotein that likely serves to regulate CRH availability to the adrenal. In human, CRHBP complexes with CRH to form a circulating 41 kDa complex. In rodent and horse, this complex is restricted to the CNS. Human CRHBP is synthesized as a 322 amino acid (aa) precursor that contains a 24 aa signal sequence and a 298 aa mature region. The mature region contains one potential CUB domain and five intrachain disulfide bonds. Human mature CRHBP shares 87% and 84% sequence identity with mouse and equine CRHBP, respectively.

Long Name

Corticotropin Releasing Hormone Binding Protein

Entrez Gene IDs

1393 (Human)

Alternate Names

corticotropin releasing hormone binding protein; corticotropin-releasing factor-binding protein; Corticotropin-releasing hormone-binding protein; CRFBP; CRFBPcorticotropin releasing hormone-binding protein; CRF-BPCRF-binding protein; CRHBP; CRH-BP

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CRHBP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Decreased CRHBP expression is predictive of poor prognosis in patients with hepatocellular carcinoma
Authors: HB Xia, HJ Wang, LQ Fu, SB Wang, L Li, GQ Ru, XL He, XM Tong, XZ Mou, DS Huang
Oncol Lett, 2018;16(3):3681-3689.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Reduced mrna expression level of corticotropin-releasing hormone-binding protein is associated with aggressive human kidney cancer.
Authors: Tezval H, Atschekzei F, Peters I, Waalkes S, Hennenlotter J, Stenzl A, Becker J, Merseburger A, Kuczyk M, Serth J
BMC Cancer, 2013;13(0):199.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot