Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 350814
View all CRISP-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CRISP-2
Lys22-Tyr243
Accession # P16562

Specificity

Detects human CRISP-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CRISP-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human CRISP‑2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CRISP‑2

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRISP-2

Human CRISP-2, the ortholog of rodent testis specific protein 1 (Tpx1) is a secreted protein expressed in testis. It is a cell adhesion molecule that mediates interactions between spermatogenic cells and Sertoli cells. Mature human CRISP-2 shares 86%, 73% and 71% amino acid sequence identity with the equine, mouse and rat proteins, respectively. Human CRISP-2 also shares 71% amino acid sequence homology with human CRISP-3.

Long Name

Cysteine-rich Secretory Protein 2

Alternate Names

CRISP2, GAPDL5, TPX1, TSP1

Entrez Gene IDs

7180 (Human); 22024 (Mouse)

Gene Symbol

CRISP2

UniProt

P16562

Additional CRISP-2 Products

Product Documents for Human CRISP‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CRISP‑2 Antibody

For research use only

Citations for Human CRISP‑2 Antibody

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