Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 755315
View all CRTAC1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CRTAC1
Ser28-Cys661
Accession # Q9NQ79

Specificity

Detects human CRTAC1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CRTAC1 Antibody

CRTAC1 antibody in Human Chondrocytes by Immunocytochemistry (ICC).

CRTAC1 in Human Chondrocytes.

CRTAC1 was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Mouse Anti-Human CRTAC1 Monoclonal Antibody (Catalog # MAB5234) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human CRTAC1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human mesenchymal stem cells differentiated into chondrocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRTAC1

CRTAC1 (cartilage acidic protein 1; also CEP-68) is a 95‑105 kDa member of a novel family of EGF domain-containing proteins. It is secreted by articular chondrocytes and may play a role in either cartilage matrix organization or cell-matrix adhesion. Mature human CRTAC1 is 634 amino acids (aa) in length. It contains four FG-GAP (PheGly-GlyAlaPro) domains (aa 46‑437) and one EGF-like motif (aa 559‑605). Multiple splice forms exist. There are two alternate start sites at Met9 and Met211 that may be accompanied by a 39 aa substitution for the C-terminal 55 aa, or an 84 aa substitution for aa 545‑661. Over aa 28‑661, human CRTAC1 shares 91% aa identity with mouse CRTAC1. This form in mouse, however, is more equivalent to the human isoform that shows a C-terminal 39 aa substitution. In this case, there is 95% aa identity between mouse and human.

Long Name

Cartilage Acidic Protein 1

Alternate Names

ASPIC1, CEP68

Entrez Gene IDs

55118 (Human); 72832 (Mouse); 171438 (Rat)

Gene Symbol

CRTAC1

UniProt

Q9NQ79

Additional CRTAC1 Products

Product Documents for Human CRTAC1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CRTAC1 Antibody

For research use only

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