Human CRTAC1 Antibody

Catalog # Availability Size / Price Qty
MAB5234
MAB5234-SP
CRTAC1 in Human Chondrocytes.
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Human CRTAC1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CRTAC1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 755315
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CRTAC1
Ser28-Cys661
Accession # Q9NQ79
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry CRTAC1 antibody in Human Chondrocytes by Immunocytochemistry (ICC). View Larger

CRTAC1 in Human Chondrocytes. CRTAC1 was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Mouse Anti-Human CRTAC1 Monoclonal Antibody (Catalog # MAB5234) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRTAC1

CRTAC1 (cartilage acidic protein 1; also CEP-68) is a 95‑105 kDa member of a novel family of EGF domain-containing proteins. It is secreted by articular chondrocytes and may play a role in either cartilage matrix organization or cell-matrix adhesion. Mature human CRTAC1 is 634 amino acids (aa) in length. It contains four FG-GAP (PheGly-GlyAlaPro) domains (aa 46‑437) and one EGF-like motif (aa 559‑605). Multiple splice forms exist. There are two alternate start sites at Met9 and Met211 that may be accompanied by a 39 aa substitution for the C-terminal 55 aa, or an 84 aa substitution for aa 545‑661. Over aa 28‑661, human CRTAC1 shares 91% aa identity with mouse CRTAC1. This form in mouse, however, is more equivalent to the human isoform that shows a C-terminal 39 aa substitution. In this case, there is 95% aa identity between mouse and human.

Long Name
Cartilage Acidic Protein 1
Entrez Gene IDs
55118 (Human); 72832 (Mouse); 171438 (Rat)
Alternate Names
ASPIC; ASPIC1; ASPIC1CEP-68acidic secreted protein in cartilage; cartilage acidic protein 1; CEP68; chondrocyte expressed protein 68 kDa CEP-68,68 kDa chondrocyte-expressed protein; CRTAC1; FLJ10320

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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