Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CSL Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CSL
Gly208-Ser500
Accession # Q06330

Specificity

Detects human CSL in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CSL Antibody

CSL in U937 Human Cell Line.

CSL was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human CSL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4079) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human CSL Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human CSL

Reviewed Applications

Read 1 review rated 5 using AF4079 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CSL

CSL (CBF1/RBP-Jk; Su(H)/Suppressor of Hairless; Lag-1) is a 56 kDa member of the Su(H) family of transcription factors. It is constitutively bound to DNA where it participates in gene repression. Upon ligand binding, the intracellular domain of Notch translocates to the nucleus and interacts with CSL, allowing transcription of Notch target genes. Human CSL is 500 amino acids (aa) in length. It contains three DNA binding sites (aa 58‑65; 192‑201; and 265‑297) and one IPT domain (aa 355‑445). Multiple splice variants exist that involve the first 89 N-terminal amino acids. One variant (isoform 2) is 487 aa in length and shows a 6 aa substitution for amino acids 1‑19. Over amino acids 195‑487, human CSL isoform 2 shares more than 98% aa sequence identity with mouse, rat, and canine CSL.

Long Name

Recombining Binding Protein Supressor of Hairless [CBF1, CSL]

Alternate Names

CBF1, IGKJRB1, KBF2, RBP-JK, RBPJ, RBPSUH

Entrez Gene IDs

3516 (Human); 19664 (Mouse)

Gene Symbol

RBPJ

UniProt

Q06330

Additional CSL Products

Product Documents for Human CSL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CSL Antibody

For research use only

Related Research Areas

Dendritic Cell Development
Notch Pathway

Customer Reviews for Human CSL Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human CSL Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human CSL Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Bladder tissue
    Species: Human
    Verified Customer | Posted 02/08/2018
    Human T24 bladder cancer cell lines, overnight incubation at 4C. Concentration used 1:500. Secondary antigoat alexa 594, 1h incubation at room temperature.
    Human CSL Antibody AF4079

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies