Human CXCR1/IL-8RA MAb (Clone 42705)

Clone 42705 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB330-SP
MAB330-100
MAB330-500
Human CXCR1/IL-8RA MAb (Clone 42705) in Flow Cytometry
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Product Details
Citations (38)
FAQs
Supplemental Products
Reviews (2)

Human CXCR1/IL-8RA MAb (Clone 42705) Summary

Species Reactivity
Human
Specificity
Detects human CXCR1/IL-8 RA transfectants but not the parental cell line. It does not cross-react with human CXCR2.
Source
Monoclonal Mouse IgG2A Clone # 42705
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR1/IL-8 RA
Met1-Leu350
Accession # AAA59159
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-reported
Cheng, Y. et al. (2016) J. Immunol. 196: 924. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize CXCL8/IL‑8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR1. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 1 ng/mL Recombinant Human CXCL8/IL‑8.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CXCR1/IL‑8 RA in Human Blood Granulocytes by Flow Cytometry. View Larger

Detection of CXCR1/IL‑8 RA in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunohistochemistry CXCR1/IL‑8 RA in Human Endometrial Cancer Tissue. View Larger

CXCR1/IL‑8 RA in Human Endometrial Cancer Tissue. CXCR1/IL-8 RA was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry CXCR1/IL‑8 RA in Human Lymph Nodes. View Larger

CXCR1/IL‑8 RA in Human Lymph Nodes. CXCR1/IL-8 RA was detected in immersion fixed frozen sections of human lymph nodes using Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to lymphocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Neutralization Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Human CXCR1/IL‑8 RA Antibody. View Larger

Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Human CXCR1/IL‑8 RA Antibody. Recombinant Human CXCL8/IL-8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL8/IL-8 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB330). The ND50is typically 0.4-2.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR1/IL-8RA

The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.

Long Name
Interleukin 8 Receptor A
Entrez Gene IDs
3577 (Human); 227288 (Mouse); 54258 (Rat)
Alternate Names
CD128; CD181 antigen; CD181; CDw128aC-C; chemokine (C-X-C motif) receptor 1; CKR-1; CMKAR1; C-X-C chemokine receptor type 1; CXCR1; CXC-R1; CXCR-1; High affinity interleukin-8 receptor A; IL-8 RA; IL-8 receptor type 1; IL-8R A; IL8R1; IL8RA; IL8RAC-C-CKR-1; IL8RBA; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A

Product Datasheets

Citations for Human CXCR1/IL-8RA MAb (Clone 42705)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming
    Authors: AJ Hoogendijk, F Pourfarzad, CEM Aarts, ATJ Tool, IH Hiemstra, L Grassi, M Frontini, AB Meijer, M van den Bi, TW Kuijpers
    Cell Rep, 2019;29(8):2505-2519.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Mesothelial Cell HIF1alpha Expression Is Metabolically Downregulated by Metformin to Prevent Oncogenic Tumor-Stromal Crosstalk
    Authors: PC Hart, HA Kenny, N Grassl, KM Watters, LM Litchfield, F Coscia, I Blaženovi?, L Ploetzky, O Fiehn, M Mann, E Lengyel, IL Romero
    Cell Rep, 2019;29(12):4086-4098.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3)
    Authors: F Caccuri, A Bugatti, S Corbellini, S Roversi, A Zani, P Mazzuca, S Marsico, A Caruso, C Giagulli
    Int J Mol Sci, 2019;20(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. IL-8/CXCR1/2 signalling promotes tumor cell proliferation, invasion and vascular mimicry in glioblastoma
    Authors: I Sharma, A Singh, F Siraj, S Saxena
    J. Biomed. Sci., 2018;25(1):62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Altered monocyte differentiation and macrophage polarization patterns in patients with breast cancer
    Authors: CH Hung, FM Chen, YC Lin, ML Tsai, SL Wang, YC Chen, YT Chen, MF Hou
    BMC Cancer, 2018;18(1):366.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients
    Authors: ALT Loi, S Hoonhorst, C van Aalst, J Langereis, V Kamp, S Sluis-Eisi, N Ten Hacken, JW Lammers, L Koenderman
    Respir. Res., 2017;18(1):100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Role of autophagy in the HIV-1 matrix protein p17-driven lymphangiogenesis
    Authors: P Mazzuca, S Marsico, K Schulze, S Mitola, MC Pils, C Giagulli, CA Guzman, A Caruso, F Caccuri
    J. Virol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Mycobacteria Manipulate G-Protein-Coupled Receptors to Increase Mucosal Rac1 Expression in the Lungs
    Authors: Nader Alaridah
    J Innate Immun, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines.
    Authors: Al-Alwan L, Chang Y, Rousseau S, Martin J, Eidelman D, Hamid Q
    J Immunol, 2015;193(3):1416-26.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Simian immunodeficiency virus and human immunodeficiency virus type 1 matrix proteins specify different capabilities to modulate B cell growth.
    Authors: Caccuri, Francesc, Giagulli, Cinzia, Reichelt, Joachim, Martorelli, Debora, Marsico, Stefania, Bugatti, Antonell, Barone, Ines, Rusnati, Marco, Guzman, Carlos A, Dolcetti, Riccardo, Caruso, Arnaldo
    J Virol, 2014;88(10):5706-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Expansion of activated regulatory T cells by myeloid-specific chemokines via an alternative pathway in CSF of bacterial meningitis patients.
    Authors: Shi G, Han J, Liu G, Hao Y, Ma Y, Li T, Wu X, Zhang H, Liu Y, Wang B, Kong Y, Zhou J, Zeng H
    Eur J Immunol, 2014;44(2):420-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Chinese herbal medicine suppresses invasion-promoting capacity of cancer-associated fibroblasts in pancreatic cancer.
    Authors: Chen, Lianyu, Qu, Chao, Chen, Hao, Xu, Litao, Qi, Qi, Luo, Jianmin, Wang, Kun, Meng, Zhiqiang, Chen, Zhen, Wang, Peng, Liu, Luming
    PLoS ONE, 2014;9(4):e96177.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. The IL-8/CXCR1 axis is associated with cancer stem cell-like properties and correlates with clinical prognosis in human pancreatic cancer cases.
    Authors: Chen, Lianyu, Fan, Jie, Chen, Hao, Meng, Zhiqiang, Chen, Zhen, Wang, Peng, Liu, Luming
    Sci Rep, 2014;4(0):5911.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. The endothelin-integrin axis is involved in macrophage-induced breast cancer cell chemotactic interactions with endothelial cells.
    Authors: Chen C, Chen L, Hsu Y, Liu K, Fan C, Huang T
    J Biol Chem, 2014;289(14):10029-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Orphan receptor GPR15/BOB is up-regulated in rheumatoid arthritis.
    Authors: Cartwright A, Schmutz C, Askari A, Kuiper J, Middleton J
    Cytokine, 2014;67(2):53-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. CXCL1/CXCL8 (GROalpha/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro.
    Authors: Omatsu T, Cepinskas G, Clarson C, Patterson E, Alharfi I, Summers K, Couraud P, Romero I, Weksler B, Fraser D
    Am J Physiol Endocrinol Metab, 2014;306(9):E1077-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche.
    Authors: Infanger, David W, Cho, YouJin, Lopez, Brina S, Mohanan, Sunish, Liu, S Chris, Gursel, Demirkan, Boockvar, John A, Fischbach, Claudia
    Cancer Res, 2013;73(23):7079-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  18. Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
    Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E
    Nat. Med., 2011;17(11):1498-503.
    Species: Human
    Sample Types: In Vivo
    Applications: In Vivo
  19. Reduced expression of chemoattractant receptors by polymorphonuclear leukocytes in Hyper IgE Syndrome patients.
    Authors: Mintz R, Garty BZ, Meshel T, Marcus N, Katanov C, Cohen-Hillel E, Ben-Baruch A
    Immunol. Lett., 2010;130(1):97-106.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6.
    Authors: Eikawa S, Ohue Y, Kitaoka K
    J. Immunol., 2010;185(11):6734-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis.
    Authors: van der Veen BS, Petersen AH, Belperio JA, Satchell SC, Mathieson PW, Molema G, Heeringa P
    Clin. Exp. Immunol., 2009;158(1):143-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. CXCR1 and CXCR2 enhances human melanoma tumourigenesis, growth and invasion.
    Authors: Singh S, Nannuru KC, Sadanandam A, Varney ML, Singh RK
    Br. J. Cancer, 2009;100(10):1638-46.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina.
    Authors: Goczalik I, Ulbricht E, Hollborn M, Raap M, Uhlmann S, Weick M, Pannicke T, Wiedemann P, Bringmann A, Reichenbach A, Francke M
    Invest. Ophthalmol. Vis. Sci., 2008;49(10):4578-89.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. Potential role of high-mobility group box 1 in cystic fibrosis airway disease.
    Authors: Rowe SM, Jackson PL, Liu G, Hardison M, Livraghi A, Solomon GM, McQuaid DB, Noerager BD, Gaggar A, Clancy JP, O'Neal W, Sorscher EJ, Abraham E, Blalock JE
    Am. J. Respir. Crit. Care Med., 2008;178(8):822-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. A novel proteolytic cascade generates an extracellular matrix-derived chemoattractant in chronic neutrophilic inflammation.
    Authors: Gaggar A, Jackson PL, Noerager BD, O'Reilly PJ, McQuaid DB, Rowe SM, Clancy JP, Blalock JE
    J. Immunol., 2008;180(8):5662-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Role of interleukin-8 and growth-regulated oncogene-alpha in the chemotactic migration of all-trans retinoic acid-treated promyelocytic leukemic cells toward alveolar epithelial cells.
    Authors: Tsai WH, Hsu HC, Lin CC, Ho CK, Kou YR
    Crit. Care Med., 2007;35(3):879-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. Cytokine and chemokine profiles in multiple myeloma; significance of stromal interaction and correlation of IL-8 production with disease progression.
    Authors: Kline M, Donovan K, Wellik L, Lust C, Jin W, Moon-Tasson L, Xiong Y, Witzig TE, Kumar S, Rajkumar SV, Lust JA
    Leuk. Res., 2007;31(5):591-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Shear-induced capping of L-selectin on the neutrophil surface during centrifugation.
    Authors: Lee D, King MR
    J. Immunol. Methods, 2007;328(1):97-105.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
    Authors: Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P
    J. Am. Coll. Cardiol., 2006;48(8):1591-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  30. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  31. The activation of IL-8 receptors in cultured guinea pig Muller glial cells is modified by signals from retinal pigment epithelium.
    Authors: Malgorzata Goczalik I, Raap M, Weick M, Milenkovic I, Heidmann J, Enzmann V, Wiedemann P, Reichenbach A, Francke M
    J. Neuroimmunol., 2005;161(1):49-60.
    Species: Guinea Pig
    Sample Types: Whole Cells
    Applications: ICC
  32. Chemokine receptor expression in rat adjuvant-induced arthritis.
    Authors: Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE
    Arthritis Rheum., 2005;52(12):3718-30.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  33. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  34. Evasion from NK cell immunity by MHC class I chain-related molecules expressing colon adenocarcinoma.
    Authors: Doubrovina ES, Doubrovin MM, Vider E, Sisson RB, O&amp;amp;apos;Reilly RJ, Dupont B, Vyas YM
    J. Immunol., 2003;171(12):6891-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003;103(7):2806-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  36. Expression of functional CCR and CXCR chemokine receptors in podocytes.
    Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
    J. Immunol., 2002;168(12):6244-52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  37. Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8.
    Authors: Liu L, Xie F, Chang K, Li M, Meng Y, Wang X, Li H, Li D, Yu J
    Int J Clin Exp Pathol, 0;7(2):575-83.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  38. IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells.
    Authors: Weiss N, Deboux C, Chaverot N, Miller F, Baron-Van Evercooren A, Couraud P, Cazaubon S
    J Neuroimmunol, 0;223(1):131-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human CXCR1/IL-8RA MAb (Clone 42705)

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Human CXCR1/IL-8 RA Antibody
By Anonymous on 04/26/2017
Application: B/N Sample Tested: Peripheral blood neutrophils Species: Human

Human CXCR1/IL-8 RA Antibody
By Anonymous on 01/23/2017
Application: IHC Sample Tested: First trimester decidua Species: Mouse

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