hh Human CXCR2/IL-8 RB Antibody MAB331-100: R&D Systems

Human CXCR2/IL-8 RB Antibody

Clone 48311 was used by HLDA to establish CD designation.
(38 citations)
(3 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCR2/IL-8 RB transfected NS0 cells but not the parental cell line. It does not cross-react with CXCR1 (IL-8 RA).
  • Source
    Monoclonal Mouse IgG2A Clone # 48311
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CXCR2/IL-8 RB
    Met1-Leu355
    Accession # AAB25880
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 ng/mL Recombinant Human CXCL1/GRO alpha /KC/CINC‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCR2/IL‑8 RB in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human
CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunohistochemistry
CXCR2/IL‑8 RB in Human Lymph Node. CXCR2/IL‑8 RB was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Mouse Anti-Human CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB331) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCR2/IL‑8 RB Antibody. Recombinant Human CXCL1/GRO alpha (Catalog #
275-GR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL1/GRO alpha (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human
CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB331). The ND50 is typically 1-5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR2/IL-8 RB
CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.
  • Long Name:
    Interleukin 8 Receptor B
  • Entrez Gene IDs:
    3579 (Human); 12765 (Mouse); 29385 (Rat)
  • Alternate Names:
    beta; CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXCR2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; IL-8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Clinicopathologic significance of the CXCL1-CXCR2 axis in the tumor microenvironment of gastric carcinoma
    Authors: H Kasashima, M Yashiro, H Nakamae, G Masuda, H Kinoshita, T Morisaki, T Fukuoka, T Hasegawa, T Nakane, M Hino, K Hirakawa, M Ohira
    PLoS ONE, 2017;12(6):e0178635.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  2. Adoptive Transfer of Phosphoantigen-Specific ?? T Cell Subset Attenuates Mycobacterium tuberculosis Infection in Nonhuman Primates
    Authors: A Qaqish, D Huang, CY Chen, Z Zhang, R Wang, S Li, E Yang, Y Lu, MH Larsen, WR Jacobs, L Qian, J Frencher, L Shen, ZW Chen
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients
    Authors: ALT Loi, S Hoonhorst, C van Aalst, J Langereis, V Kamp, S Sluis-Eisi, N Ten Hacken, JW Lammers, L Koenderman
    Respir. Res., 2017;18(1):100.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression
    Authors: WW Xu, B Li, XY Guan, SK Chung, Y Wang, YL Yip, SY Law, KT Chan, NP Lee, KW Chan, LY Xu, EM Li, SW Tsao, QY He, AL Cheung
    Nat Commun, 2017;8(0):14399.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Evidence for chemokine synergy during neutrophil migration in ARDS
    Thorax, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. TNF-? augments CXCR2 and CXCR3 to promote progression of renal cell carcinoma
    Authors: Kuang-Hui Sun
    J Cell Mol Med, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines.
    Authors: Al-Alwan L, Chang Y, Rousseau S, Martin J, Eidelman D, Hamid Q
    J Immunol, 2015;193(3):1416-26.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. CXCR2-CXCL1 axis is correlated with neutrophil infiltration and predicts a poor prognosis in hepatocellular carcinoma.
    Authors: Li L, Xu L, Yan J, Zhen Z, Ji Y, Liu C, Lau W, Zheng L, Xu J
    J Exp Clin Cancer Res, 2015;34(0):129.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  9. CXCL1/CXCL8 (GROalpha/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro.
    Authors: Omatsu T, Cepinskas G, Clarson C, Patterson E, Alharfi I, Summers K, Couraud P, Romero I, Weksler B, Fraser D
    Am J Physiol Endocrinol Metab, 2014;306(9):E1077-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  10. Expansion of activated regulatory T cells by myeloid-specific chemokines via an alternative pathway in CSF of bacterial meningitis patients.
    Authors: Shi G, Han J, Liu G, Hao Y, Ma Y, Li T, Wu X, Zhang H, Liu Y, Wang B, Kong Y, Zhou J, Zeng H
    Eur J Immunol, 2014;44(2):420-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph.
    Authors: Carrega, Paolo, Bonaccorsi, Irene, Di Carlo, Emma, Morandi, Barbara, Paul, Petra, Rizzello, Valeria, Cipollone, Giuseppe, Navarra, Giuseppe, Mingari, Maria Cr, Moretta, Lorenzo, Ferlazzo, Guido
    J Immunol, 2014;192(8):3805-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Simian immunodeficiency virus and human immunodeficiency virus type 1 matrix proteins specify different capabilities to modulate B cell growth.
    Authors: Caccuri, Francesc, Giagulli, Cinzia, Reichelt, Joachim, Martorelli, Debora, Marsico, Stefania, Bugatti, Antonell, Barone, Ines, Rusnati, Marco, Guzman, Carlos A, Dolcetti, Riccardo, Caruso, Arnaldo
    J Virol, 2014;88(10):5706-17.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  13. The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma.
    Authors: Stofas A, Levidou G, Piperi C, Adamopoulos C, Dalagiorgou G, Bamias A, Karadimou A, Lainakis G, Papadoukakis S, Stravodimos K, Dimopoulos M, Patsouris E, Gakiopoulou H, Korkolopoulou P
    BMC Cancer, 2014;14(0):149.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  14. Tumor-derived interleukin-1 promotes lymphangiogenesis and lymph node metastasis through M2-type macrophages.
    Authors: Watari K, Shibata T, Kawahara A, Sata K, Nabeshima H, Shinoda A, Abe H, Azuma K, Murakami Y, Izumi H, Takahashi T, Kage M, Kuwano M, Ono M
    PLoS ONE, 2014;9(6):e99568.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  15. Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche.
    Authors: Infanger, David W, Cho, YouJin, Lopez, Brina S, Mohanan, Sunish, Liu, S Chris, Gursel, Demirkan, Boockvar, John A, Fischbach, Claudia
    Cancer Res, 2013;73(23):7079-89.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  16. Overexpression of the beta subunit of human chorionic gonadotropin promotes the transformation of human ovarian epithelial cells and ovarian tumorigenesis.
    Authors: Guo X, Liu G, Schauer IG, Yang G, Mercado-Uribe I, Yang F, Zhang S, He Y, Liu J
    Am. J. Pathol., 2011;179(3):1385-93.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  17. Overexpression of CXCL5 is associated with poor survival in patients with pancreatic cancer.
    Authors: Li A, King J, Moro A, Sugi MD, Dawson DW, Kaplan J, Li G, Lu X, Strieter RM, Burdick M, Go VL, Reber HA, Eibl G, Hines OJ
    Am. J. Pathol., 2011;178(3):1340-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  18. Interleukin-33 overexpression is associated with gamma-glutamyl transferase in biliary atresia.
    Cytokine, 2011;61(2):433-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  19. Reduced expression of chemoattractant receptors by polymorphonuclear leukocytes in Hyper IgE Syndrome patients.
    Authors: Mintz R, Garty BZ, Meshel T, Marcus N, Katanov C, Cohen-Hillel E, Ben-Baruch A
    Immunol. Lett., 2010;130(1):97-106.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  20. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2010;84(3):239-51.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  21. CXCR2 promotes ovarian cancer growth through dysregulated cell cycle, diminished apoptosis, and enhanced angiogenesis.
    Authors: Yang G, Rosen DG, Liu G
    Clin. Cancer Res., 2010;16(15):3875-86.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  22. Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis.
    Authors: van der Veen BS, Petersen AH, Belperio JA, Satchell SC, Mathieson PW, Molema G, Heeringa P
    Clin. Exp. Immunol., 2009;158(1):143-53.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  23. CXCR1 and CXCR2 enhances human melanoma tumourigenesis, growth and invasion.
    Authors: Singh S, Nannuru KC, Sadanandam A, Varney ML, Singh RK
    Br. J. Cancer, 2009;100(10):1638-46.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  24. Potential role of high-mobility group box 1 in cystic fibrosis airway disease.
    Authors: Rowe SM, Jackson PL, Liu G, Hardison M, Livraghi A, Solomon GM, McQuaid DB, Noerager BD, Gaggar A, Clancy JP, O'Neal W, Sorscher EJ, Abraham E, Blalock JE
    Am. J. Respir. Crit. Care Med., 2008;178(8):822-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  25. A novel proteolytic cascade generates an extracellular matrix-derived chemoattractant in chronic neutrophilic inflammation.
    Authors: Gaggar A, Jackson PL, Noerager BD, O'Reilly PJ, McQuaid DB, Rowe SM, Clancy JP, Blalock JE
    J. Immunol., 2008;180(8):5662-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  26. Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina.
    Authors: Goczalik I, Ulbricht E, Hollborn M, Raap M, Uhlmann S, Weick M, Pannicke T, Wiedemann P, Bringmann A, Reichenbach A, Francke M
    Invest. Ophthalmol. Vis. Sci., 2008;49(10):4578-89.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Fresh
  27. Synergy between coproduced CC and CXC chemokines in monocyte chemotaxis through receptor-mediated events.
    Authors: Gouwy M, Struyf S, Noppen S, Schutyser E, Springael JY, Parmentier M, Proost P, Van Damme J
    Mol. Pharmacol., 2008;74(2):485-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  28. TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche.
    Authors: Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H
    Cancer Res., 2008;68(23):9754-62.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  29. Cytokine and chemokine profiles in multiple myeloma; significance of stromal interaction and correlation of IL-8 production with disease progression.
    Authors: Kline M, Donovan K, Wellik L, Lust C, Jin W, Moon-Tasson L, Xiong Y, Witzig TE, Kumar S, Rajkumar SV, Lust JA
    Leuk. Res., 2007;31(5):591-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  30. Role of interleukin-8 and growth-regulated oncogene-alpha in the chemotactic migration of all-trans retinoic acid-treated promyelocytic leukemic cells toward alveolar epithelial cells.
    Authors: Tsai WH, Hsu HC, Lin CC, Ho CK, Kou YR
    Crit. Care Med., 2007;35(3):879-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  31. Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
    Authors: Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P
    J. Am. Coll. Cardiol., 2006;48(8):1591-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  32. The activation of IL-8 receptors in cultured guinea pig Muller glial cells is modified by signals from retinal pigment epithelium.
    Authors: Malgorzata Goczalik I, Raap M, Weick M, Milenkovic I, Heidmann J, Enzmann V, Wiedemann P, Reichenbach A, Francke M
    J. Neuroimmunol., 2005;161(1):49-60.
    Species: Guinea Pig
    Sample Type: Whole Cells
    Application: ICC
  33. Chemokine receptor expression in rat adjuvant-induced arthritis.
    Authors: Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE
    Arthritis Rheum., 2005;52(12):3718-30.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  34. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Neut
  35. Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2.
    Authors: Traves SL, Smith SJ, Barnes PJ, Donnelly LE
    J. Leukoc. Biol., 2004;76(2):441-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  36. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003;103(7):2806-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  37. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
    Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
    J. Immunol., 2002;169(11):6515-21.
    Species: Human
    Sample Type: BALF
    Application: ELISA Development
  38. Expression of functional CCR and CXCR chemokine receptors in podocytes.
    Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
    J. Immunol., 2002;168(12):6244-52.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
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Average Rating: 4.3 (Based on 3 reviews)

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Read what people are saying who have used Human CXCR2/IL-8 RB Antibody.

We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 reviews tested in 3 applications: Block/Neutralize, Immunohistochemistry, Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
  Very Good
 Block/Neutralize Human Anonymous 04/26/2017
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Summary

ApplicationBlock/Neutralize
Sample TestedPeripheral blood neutrophils
SpeciesHuman
Immunohistochemistry Human CXCR2/IL-8 RB Antibody MAB331
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Very Good
 IHC Mouse Anonymous 01/23/2017
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Immunohistochemistry Human CXCR2/IL-8 RB Antibody MAB331
Immunohistochemistry: Human CXCR2/IL-8 RB Antibody [MAB331]

Summary

ApplicationImmunohistochemistry
Sample Testedfetal membrane
SpeciesMouse

Other Experimental Details

Other Experimental Detailssodium citrate antigen retrieval
Immunocytochemistry/Immunofluorescence Human CXCR2/IL-8 RB Antibody MAB331
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 ICC/IF Human Anonymous 11/04/2016
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Immunocytochemistry/Immunofluorescence Human CXCR2/IL-8 RB Antibody MAB331
Immunocytochemistry/Immunofluorescence: Human CXCR2/IL-8 RB Antibody [MAB331]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedAdult lung
SpeciesHuman

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