Human CXCR6 Antibody Summary
Accession # O00574
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CXCR6 in Human PBMCs Stimulated to Induce Th1 Cells by Flow Cytometry. Human CD3+peripheral blood mononuclear cells (PBMCs) cultured with plate bound anti-Human CD3 and CD28 Antibodies, Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA; 20 µg/mL) and 10 ng/mL Recombinant Human IL-12 (Catalog # 219-IL) for 5 days to induce Th1 cell development were stained with (A) Mouse Anti-Human CXCR6 Monoclonal Antibody (Catalog # MAB6991) or (B) Isotype control antibody (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CXCR6, also known as BONZO, STRL33, TYMSTR, is the receptor for the C-X-C chemokine CXCL16. CXCR6 is a G protein coupled protein receptor (GPCR) expressed on activated T cells and tumor-infiltrating lymphocytes, and it has been identified as an entry co-receptor used by HIV-1 and SIV to enter target cells, in conjunction with CD4.
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