Detects human Cyclin D3 in direct ELISAs.
In direct ELISAs and Western blots,
approximately 5% cross-reactivity with recombinant human (rh) Cyclin B1 and D1
is observed. No cross-reactivity with rhCyclin A1, A2, B2, C, D2, E1, or E2 is
Monoclonal Mouse IgG2B Clone # 588726
Protein A or G purified from hybridoma culture supernatant
E.coli-derived recombinant human Cyclin D3 Met1-Leu292 Accession # P30281
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse Cyclin D3 by Western Blot. Western blot shows lysates of BaF3 mouse pro-B cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human Cyclin D3 Monoclonal Antibody (Catalog # MAB6570) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cyclin D3 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cyclin D3 in Human Breast Cancer Tissue. Cyclin D3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human Cyclin D3 Monoclonal Antibody (Catalog # MAB6570) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyclin D3
Cyclin D3 is a 37 kDa cell cycle regulatory protein whose
expression level and nuclear/cytoplasmic distribution are tightly regulated in
synchrony with the cell cycle. Cyclin D3 complexes containing Cdk4 or Cdk6
induce phosphorylation of Rb, a requirement for progression through the G1/S
cell cycle transition. Human Cyclin D3 shares 95% and 93% amino acid sequence
identity with mouse and rat Cyclin D3, respectively.
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