Cystatin C is a member of family 2 of the Cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). It is ubiquitous in human tissues and body fluids. A point mutation in the gene coding for the 120 amino acid mature Cystatin C causes a hereditary form of amyloid angiopathy in which the protein variant (Leu68 to Gln) is deposited in the cerebral arteries, leading to fatal cerebral hemorrhage (4). Cystatin C may have additional clinical applications. For example, it is a good marker for glomerular filtration rate (5).
Human Cystatin C Antibody (197820)
R&D Systems | Catalog # MAB11962
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 197820
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cystatin C
Ser27-Ala146
Accession # P01034
Ser27-Ala146
Accession # P01034
Specificity
Detects human Cystatin C in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human Cystatins A, B, D, E/M, F, S, SA, SN, X/Z/P, or recombinant mouse Cystatin C is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Cystatin C Antibody (197820)
Human Cystatin C ELISA Standard Curve
Recombinant Human Cystatin C (Catalog # 1196-PI) was serially diluted and captured by Mouse Anti-Human Cystatin C Monoclonal Antibody (Catalog # MAB11962) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Cystatin C Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human Cystatin C Antibody (197820)
Human Cystatin C Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB11962 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Cystatin C
References
- Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
- Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Abrahamson, M. et al. (1992) Hum. Genet. 89:377.
- Laterza, O.F. et al. (2002) Clin. Chem. 48:699.
Alternate Names
ARMD11, CST3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin
Gene Symbol
CST3
UniProt
Additional Cystatin C Products
Product Documents for Human Cystatin C Antibody (197820)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Cystatin C Antibody (197820)
For research use only
Related Research Areas
Citations for Human Cystatin C Antibody (197820)
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5 out of 5
2 Customer Ratings
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Application: ImmunohistochemistrySample Tested: Alzheimer's disease brainSpecies: HumanVerified Customer | Posted 06/07/2018Cystatin C was detected in human brain endothelial cells (formalin fixed tissue)
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Application: ELISASample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 03/15/2018
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