Human Cytokeratin 18 Antibody Summary
Accession # P05783
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Cytokeratin 18 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cytokeratin 18 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Cytokeratin 18 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line (Cytokeratin 18 positive) and MOLT‑4 human acute lymphoblastic leukemia cell line (Cytokeratin 18 negative). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Cytokeratin 18 at approximately 45 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Cytokeratin 18 in HeLa Human Cell Line. Cytokeratin 18 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to intermediate filaments in cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Cytokeratin 18 in Human Squamous Cell Carcinoma. Cytokeratin 18 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Cytokeratin 18 by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Cytokeratin 18 at approximately 55 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytokeratin 18
Cytokeratin 18; also KRT-18 (Keratin, type I cytoskeletal 18), Cell proliferation-inducing gene 46 and Keratin-18) is a 44-48 kDa Class I (large keratins of acidic pH) member of the intermediate filament family of proteins. Individual keratins are always expressed in tandem with a second keratin, and these are found in all epithelial cells. The class I Cytokeratin 18 heterodimerizes/polymerizes with 50-52 kDa class II KRT-8 to form 8-10 nm filaments in single strata plus hepatic epithelia. Cytokeratin 18 and -8 are the first keratins to appear in the mammalian embyro. In the adult, Cytokeratin 18 appears to participate in subtractions and additions to the plasma membrane. In this regard, a number of intracellular proteins interact with Cytokeratin 18, including 14-3-3, HSPc70 and Mrj. Cytokeratin 18 may also be O‑glycosylated, and when so, serves to promote Akt-1 activity, thus protecting against apoptosis. Human Cytokeratin 18 is 430 amino acids (aa) in length. It contains an N-terminal "head" region (aa 1-79), a subsequent "rod" region (aa 80-387) with two coiled segments, and a C-terminal tail region. Cytokeratin 18 possesses at least 19 utilized phosphorylation sites plus five acetylated Lys residues. There are multiple isoforms that range from 20-40 kDa in size and are the result of caspase cleavage. A principal cleavage site occurs after Asp238. Over aa 239-397, human Cytokeratin 18 shares 86% aa sequence identity with mouse Cytokeratin 18.
Citation for Human Cytokeratin 18 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
TGF?1 in fibroblasts-derived exosomes promotes epithelial-mesenchymal transition of ovarian cancer cells
Authors: W Li, X Zhang, J Wang, M Li, C Cao, J Tan, D Ma, Q Gao
Sample Types: Whole Cells
Applications: Flow Cytometry
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