Human Cytokeratin 18 Antibody (810811)

R&D Systems | Catalog # MAB7619

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 810811
View all Cytokeratin 18 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cytokeratin 18
Ala239-Asp397
Accession # P05783

Specificity

Detects human Cytokertin 18 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Cytokeratin 18 Antibody (810811)

Detection of Human Cytokeratin 18 antibody by Western Blot.

Detection of Human Cytokeratin 18 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cytokeratin 18 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Cytokeratin 18 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line (Cytokeratin 18 positive) and MOLT‑4 human acute lymphoblastic leukemia cell line (Cytokeratin 18 negative). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Cytokeratin 18 at approximately 45 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Cytokeratin 18 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Cytokeratin 18 in HeLa Human Cell Line.

Cytokeratin 18 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to intermediate filaments in cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Cytokeratin 18 antibody in Human Squamous Cell Carcinoma by Immunohistochemistry (IHC-P).

Cytokeratin 18 in Human Squamous Cell Carcinoma.

Cytokeratin 18 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Cytokeratin 18 antibody by Simple WesternTM.

Detection of Human Cytokeratin 18 by Simple WesternTM.

Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Cytokeratin 18 at approximately 55 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human Cytokeratin 18 Monoclonal Antibody (Catalog # MAB7619). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Cytokeratin 18 Antibody (810811)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded human squamous cell carcinoma

Simple Western

2 µg/mL
Sample: A431 human epithelial carcinoma cell line

Western Blot

0.2-1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and A431 human epithelial carcinoma cell line

Reviewed Applications

Read 3 reviews rated 4.3 using MAB7619 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytokeratin 18

Cytokeratin 18; also KRT-18 (Keratin, type I cytoskeletal 18), Cell proliferation-inducing gene 46 and Keratin-18) is a 44-48 kDa Class I (large keratins of acidic pH) member of the intermediate filament family of proteins. Individual keratins are always expressed in tandem with a second keratin, and these are found in all epithelial cells. The class I Cytokeratin 18 heterodimerizes/polymerizes with 50-52 kDa class II KRT-8 to form 8-10 nm filaments in single strata plus hepatic epithelia. Cytokeratin 18 and -8 are the first keratins to appear in the mammalian embyro. In the adult, Cytokeratin 18 appears to participate in subtractions and additions to the plasma membrane. In this regard, a number of intracellular proteins interact with Cytokeratin 18, including 14-3-3, HSPc70 and Mrj. Cytokeratin 18 may also be O‑glycosylated, and when so, serves to promote Akt-1 activity, thus protecting against apoptosis. Human Cytokeratin 18 is 430 amino acids (aa) in length. It contains an N-terminal "head" region (aa 1-79), a subsequent "rod" region (aa 80-387) with two coiled segments, and a C-terminal tail region. Cytokeratin 18 possesses at least 19 utilized phosphorylation sites plus five acetylated Lys residues. There are multiple isoforms that range from 20-40 kDa in size and are the result of caspase cleavage. A principal cleavage site occurs after Asp238. Over aa 239-397, human Cytokeratin 18 shares 86% aa sequence identity with mouse Cytokeratin 18.

Alternate Names

CK-18, CYK18, Keratin 18, KRT18

Entrez Gene IDs

3875 (Human); 16668 (Mouse)

Gene Symbol

KRT18

UniProt

P05783 (Human)

Additional Cytokeratin 18 Products

Product Documents for Human Cytokeratin 18 Antibody (810811)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cytokeratin 18 Antibody (810811)

For research use only

Citations for Human Cytokeratin 18 Antibody (810811)

Customer Reviews for Human Cytokeratin 18 Antibody (810811) (3)

4.3 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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3 Stars
33%
2 Stars
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1 Stars
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  • Human Cytokeratin 18 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Testis tissue
    Species: Human
    Verified Customer | Posted 10/20/2021
    Human Cytokeratin 18 Antibody (810811) MAB7619
  • Human Cytokeratin 18 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Bladder cancer tissue
    Species: Human
    Verified Customer | Posted 12/19/2017
    Human Cytokeratin 18 Antibody (810811) MAB7619
  • Human Cytokeratin 18 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: pig trabecular meshwork cells
    Species: Human
    Verified Customer | Posted 11/28/2016
    Detection of cytokeratin 18 in cultured pig trabecular meshwork cells using Human Cytokeratin 18 Antibody (MAB7619) at 1:500 dilution overnight and donkey anti-mouse Alexa 594 (1:1000) for 1 hour.
    Human Cytokeratin 18 Antibody (810811) MAB7619

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